BSc Biochemistry Sem V 2014 2015 2015 Paper 3 Question Paper - Mumbai University | munotes

Questions asked in this paper

• (2) Figures to the right indicate full marks. <

1. Q1 (A) Choose the MOST appropriate option : (any three) ax
   • (i) The elongation step of replication uses as a source of energy
   • (ii) required for the process of initiation of replication
   • (iii) DNA synthesis takes place in direction
   • (iv) During mismatch repair, the sequcnce is used for of the
   • (v) UV radiations lead largely to the formation of
   • (vi) Gyrase helps in (Unwinding removing supercoils joining of fragments)
   • (B) Answer the following any one :—
   • (i) Describe the rolling circle mode of
   • (ii) Give the role of alkyl transferase in REA repair
   • (C) Write short note on any one :— 4
   • (D) Attempt any one :—
   • (i) Discuss replication
   • (ii) Enlist any two types of QNA damages that usually occur. Elaborate on the Excission repair
2. Q2 (A) Choose the MOST option : (any three) 3 marks
   • (i) Translation . (cytoplasm nucleus mitochondria)
   • (ii) In eukaryotes RNA transcript that is translated contains (both and exons only introns only exons)
   • (iii) Yox generally acts as promoter at -10 bp during transcription
   • (iv) Thexgnticodon to the mRNA codon 5’AUG is (Shine Dalgamo sequence is present on is a post translational modification
   • (B) Define Explain the terms any one :— 2
   • (C) Write short note on any one of the following :— 4
   • (i) Wobble hypothesis and Genetic Code <
   • (D) Answer in detail any one :— 6 fe
   • (i) Elaborate on the initiation and termination steps in translation
   • (ii) Discuss the various modifications that take place post-transcription
3. Q3 (A) Choose the MOST appropriate option : (any three) 3 marks
   • (i) A bacterium protects its DNA from cleavage by its own (DNA ligase quickly re-seals target sequences when they are attach to the target sequences, preventing restriction ses from binding bacterium chemically modifies its own DNA thereby preventing recognition by the nucleases) In Klenow fragment activity is
   • (iii) Type II restriction endonuclease has BS recognition site
   • (iv) DNA is generally used
   • (v) YAC is (only a cloning only a shuttle a cloning and shuttle)
   • (B) Define and explain any one 2
   • (C) Write brief notes on any 4
   • (D) Attempt any one :— 6
   • (i) Elaborate o vectors
   • (ii) Discuss genetic engineering has helped revolutionize food and
4. Q4 (A) Choose appropriate option : (any three) 3 marks
   • (i) croinjection can be used in cells protoplast both walled cells and protoplast) ts) The enzyme can work at high temperatures (ili) Southern blotting is used for separation
   • (iv) CaCl, helps in
   • (v) InLac selection, cells transformed with vectors containing recombinant Be) DNA will produce colonies. (white blue clear)
   • (vi) PCR was developed by : (Watson & Crick University of California) (B efine and explain the terms any one :—
   • (C) Write short note on any one :— 4
   • (D) Elaborate on any one :— 6 Answer any one :— 3
   • (i) Give the similarities and differences between the types of DNA
   • (ii) Discuss the role of ligase and helicase in
   • (B) Write brief notes on any one :— 3
   • (i) Inhibitors of translation
   • (ii) Charging of tRNA
   • (C) Answer in brief any one S 3
   • (i) What is role of reverse recombinant DNA technology ?
   • (ii) Write a note on radioactive
   • (D) Answer in brief on any one SY 3
   • (i) Write a brief note on
   • (ii) Schematically only the steps in involved in southern blotting
   • (E) State true or false (any 3
   • (i) DNA on both strands
   • (ii) Okazaki are formed in the leading strand
   • (iv) Genomic BNA is the starting material for making a cDNA library
   • (v) Rho is involved in termination of translation
   • (vi) 5 exhibit the lytic and the lysogenic phases of life cycle

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