BSc Biochemistry Sem V 2014 2015 2015 Paper 2 Question Paper - Mumbai University | munotes

(2 % Hours) [ Total Marks ; 75
questions are compulsory
(2) Choice is internal
(4) Non Programmable calculators are allowed
(S) Figures to the right indicate full marks
1 (@) Fillin the blanks with the most appropriate answer from the options provided 3
(i) is an imino acid
(ii) enzyme brings about cleavage of polypeptides
(iil) Proteins are not denatured by
(high temperature, low temperature, changes in pH)
(iv) An example of a contractile protein is
(v) Secondary structure of proteins is stabilized by
(vi) is a globular protein
(b) Attempt any one of the following: 2
(i) Justify how an amino acid can behave as an acid as well as a base
(ii) Name any four bonds which stabilize the tertiary structure of proteins
(c) Answer any one of the 4
(i) Effect of any two proteolytic enzymes on polypeptides
(ii) Characteristics of peptide bonds
(d) Write briefly on auy one of the following: 6
(i) Write any two complete reactions of amino acids, Give their biochemical
(ii) Functional diversity of proteins
2 (a) the blanks with the most appropriate answer from the options provided. 3
An unit of measurement of enzyme
(ii) Competitive inhibitor of enzyme succinate dehydrogenase
(iii) is a Sugar present in RNA and DNA
(iv) DNA contains all the bonds EXCEPT
(v) EC class number for ligases is
(vi) Nucleotide of adenine is called
(b) Define any one of the following: 2
(i) Prosthetic group
(ii) Nucleoside
(c) Write briefly on any one of the following: 4
(i) Effect of pH and temperature on enzyme activity
(d) Attempt any one of the following: 6
Anote on enzyme inhibition
(ii) Physical properties of DNA and the effec: ofincrease in temperature on them
(a) the blanks with the most appropriate answer from the options provided 3
(i) is a reducing sugar
(ii) Storage polysaccharide found in
(1i1) Sedoheptulose is a _ sugar,
(iv) Glycogen is a polymer made up of units,
(v) Heparin is an example of
(vi) Oxidation of glucose at the sixth carbon forrns acid
(b) Attempt any one ofthe following:
(1) Structure of sucrose
(c) Answer any one of the following:
(i) Action of alpha and beta amylase on starch
most appropriate a swer from the options provided
ution of 0.01 N hydrochloric acid
of - log, of -log,, of
0.693/Lambda,t,
radioisotope is used in detection of hypo/hyperthyroidism
used in titration
(b) Define any one of the following: 2
(i) Isoelectric pH
(ii) Radioactivity
(c) Answer any one of the following. 4
(i) Define buffer. Derive the Henderson- Hasselbalch equation
(d) Write briefly on any one the following: 6
(i) Titration curve and ionization forms of glycine
(ii) Applications of radioisotopes In biological studies
5. (a) Answer briefly any one of the following : 3
(i) Specia! features of alpha helical structure of proteins
(ii) Struviures of
Attempt any one of the following: 3
Lineweaver Burk plot and its significance
Write the structures of ATP and cAMP
Write the structure and significance of any one of the following: 3
(d) Write briefly on any one of the following: 3
(i) Glass electrode
(ii) Decay constant and half life of a radioisotope
State true or false, attempt any three : 3
(ii) Fructose is an aldose sugar
(iii) N is used in metabolic studies
(iv) Proline is a sulphur containing amino acid
(v) Aspartate transcarbamoylase is an allosteric enzyme
(vi) Peptones are derived proteins