BSc Biochemistry Sem V ATKT BIO CHEM BIOCHEMISTRY PAPER III ADVANCED GENETICS & RD5 Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q1 A) Choose the MOST appropriate answer: (any three) 3 marks
   • i) The lagging strand of DNA is synthesized in direction (Pol I; Pol II ; Pol I and ID) lil) In light thymine dimers may be formed
   • iv) undergoes deamination and becomes uracil
   • v) Replication occurs in
5. Q1 B) and explain any one of the following: 2 marks
   • i) Central Dogma Write ashort note on any one: 4
6. Q1 Excision repair li) Enzymes involved in replication
7. Q1 D) Write a detailed note on any one: 6 marks
   • i) Mechanism of replication
   • ii) Direct repair and mismatch repair the MOST appropriate answer: (any three) 3
8. Q1 DNA is not directly involved in
   • ii) nucleotide base is not found in RNA ili) With mRNA codon, the tRNA with 5'CUA3' will form a codon
   • iv) gave the Central Dogma of molecular biology
   • v) Reverse transcriptase is seen in
9. Q2 B) and explain any one of the following: 2 marks
10. Q1 Promoter sequences
11. Q2 C) Write ashort note on any one: 4 marks
12. Q1 Splicing
13. Q2 D) Write a detailed note on any one: 6 marks
14. Q1 Transcription in prokaryotes li) Translation in prokaryotes
15. Q3 A) Choose the MOST appropriate answer: (any three) li) The DNA to which the gene of interest is integrated is called a lil) popularly are called “Molecular stitchers” 3 marks
    • iv) YAC stands for American chromosome/Y east Artificial
    • v) Reverse transcriptase forms a DNA hybrid that is known as
16. Q3 B). Define and explain, any one: 2 marks
17. Q3 C) Write ashort note on any one: 4 marks
18. Q1 Significance and labelling of probes
19. Q3 D) an elaborate note on Write a short note on any one: li) Applications of RDT 6 marks
20. Q4 A) the MOST appropriate answer: (any three) 3 marks
    • i) DNA is injected through in micro projectile technique both shotgun and liposomes) li) Heating of nitrocellulose at high temperature is known as ili) Transfer of recombinant plasmid into E. coli cells
    • iv) The process of introduction of foreign DNA into an animal cells is called
    • v) Polymerase used for PCR is extracted from
21. Q4 B) Define and explain any one: 2 marks
22. Q1 Chimeric DNA
23. Q4 C) Write a short note on any one: 4 marks
24. Q1 Methods for transfer of recombinant gene into the host
    • ii) PCR
25. Q4 D) Write note on any one: li) Gene library and cDNA library 6 marks
26. Q5 brief note on any four, as directed: 12 marks
27. Q1 Modes of DNA replication
    • ii) Initiation of translation ili) Role of ligase in RDT
    • iv) Use of Liposomes in RDT
28. Q5 B) State TRUE or FALSE: (any three)
29. Q1 Restriction enzymes act only as endonucleases li) Transcription starts at origin of replication lil) Plagemids are always smaller than bacteriophages
    • iv) All Polymerase are present in the cytoplasm
    • v) Introduction of genes using a virus is termed as Transfection Vi) Rho factor is involved in initiation of Replication

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