BSc Biochemistry Sem V 2018 19 2018-19 TYBIO CHEM Biochemistry I Metabolism & Ana. & Tech. 19 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q1 B) in the blanks: (any three) 3 marks
5. Q1 is aenzyme requiring ATP as substrate li) The starting material for TCA is
   • iii) is a polymer of Iv) is a pentulose Define and explain any one 3
6. Q1 Glycogenesis Attempt any one: 6 li) Write a note on Pyruvate Dehydrogenase Enzyme complex
7. Q1 D) Attempt any one: 8 marks
8. Q1 Write notes on a) Glycogenolysis. and (b) Glyoxylate cycle li) If C3 and C4 of Glucose are labelled with C-14, trace the fate of C-14 in in the blanks: (Any three) 3
   • i) Dark reaction takes place in
   • ii) The initial electron donor of the ETC is , which gives its two electrons off to FMN, within Complex II ili) The key enzyme that accomplishes the fixing of carbon is Most abundant enzyme on earth is
9. Q2 B) — Attempt any one: 3 marks
10. Q1 Explain (a) where (b) how cyanide stops ATP production in the li) How has Hill contributed to our understanding of photosynthesis
11. Q2 C) detail answer any one: 6 marks
12. Q1 What is the significance of Malate-Aspartate Shuttle? With the help of a representation explain the same li) Explain by means of a diagram the process of ATP formation by
13. Q2 D) Answer any one in detail: 8 marks
14. Q1 Schematically represent the Z scheme with all the electron carriers li) Discuss the electron chain carriers in ETC Fill in the blanks: (any three) 3
15. Q1 Calcium carbonate can be used for chromatography li) Distribution coefficient cannot have a value lil) can be a mobile phase in GC
    • iv) Antibodies can be separated by chromatography
16. Q3 B) Define and explain any one 3 marks
17. Q3 C) Write a detailed answer for any one: 6 marks
18. Q1 Describe a technique to separate. glucose, lactose and xylose li) Discuss the principle and applications of Ion exchange chromatography
19. Q3 D) Attempt any one: 8 marks
20. Q1 Elaborate on Gel Chromatography li) Write notes on a) TLC and (b) Column Chromatography Fillin the blanks: (Any three) 3
    • i) The pH of a carbonated drink is li) A solution with pH 2 is compared to a solution of pH 4 ili) is acoenzyme for reactions
    • iv) The coenzyme form of thiamine is Attempt any one: 3
    • i) Mention the (a) coenzyme form and (b) biochemical role of riboflavin li) Justify: 'pH meter works on the principle of ion exchange'
21. Q4 C) Answer any one in detail: 6 marks
    • i) What is the visual cycle? What is the role of Vitamin A in the visual li) Derive the Henderson Hasselbalch equation Answer any one in detail: 8
22. Q1 With the help of a graph, explain the titration curve of a acidic amino acid. Mention the pKa values and the pI value as well. What is the relevance of titration curve to human physiology? li) Justify the statement: 'Vitamin D metabolism is both regulated by and
23. Q5 A) Answer in brief any four of the following: 16 marks
24. Q1 Explain giving suitable examples anapleurotic reactions
25. Q1 Discuss the reactions of Gluconeogenesis li) Discuss the significance of Calvin cycle li) Write an elaborate note on Chemiosmotic hypothesis
    • iii) the principles of Affinity chromatography and HPLC ili) briefly on the different types of paper chromatography
    • iv) a detailed note on vitamin K
    • iv) Titration curve. Draw the titration curve of glycine, with special mention of pKa values and pl
26. Q5 B) State TRUE or FALSE: (any four) li) Lysine has four ionizable groups 4 marks
    • iii) Ninhydrin is the detecting agent for visualising sugar separation through TCA is also called EMP Vi) involves expenditure of ATP

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