BSc Biochemistry Sem V 2014 2015 2015 Paper 1 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q2 (A) Fill in the blanks(any three) (ij) The NADH shuttle used by skeletal muscles and brain is_________— 3 marks
   • (ii) the dependence of electron transfer on ATP synthesis in mitochondria
   • (b) Rotational catalysis
   • (c) Chemiosmotic theory
   • (iii) results in the passage of electrons through in ETC
   • (a) Complex IV
   • (b) Complex
   • (c) Complex V
   • (iv) Iron-sulphur proteins are characteristic
   • (a) ETC
   • (c) Both ‘a’ and 'b'
   • (a) Cyanide
   • (b) Rotenone
   • (c) Amytal
   • (B) Define and explain(any one) 2
   • (i) Free energy (ii)
   • (C) Discuss in detail(any one) 4
   • (ii) | Dark reaction and light reaction of photosynthesis
   • (D) Elaborate on (any one) 6
   • (i) of photosynthesis
   • (ii) Structure of ATP synthase
   • (A) Fill in the blanks(any three) 3
   • (i) The stationary phase in reverse phase partition chromatography is
   • (c) Water ) In GLC, a stationary phase of silicon grease is supported the detection of is._ useful if unsaturated ounds are being investigated
2. Q1 Iodine vapour In TLC, for charged lipids, are used
   • i) moderately moving zones are obtained explain (any one):- 2 Application of gel filtration chromatography
   • (A) File i three): consists of sugar aleohol attached to
   • (iv) The isoelectric pH of glycine is calculated by arithmetic mean of and part as coenzyme in transamination
   • (a) Pyridoxine
   • (b) Flavin
   • (c) Biotin
   • (vi) curve, the relatively flat zone observed is
   • (a) Titration zone
   • (b) Acceptor zone
   • (c) Buffering zone
   • (B) Define and Explain the following(any one) 2
   • (i) Vitamins
   • (C) Write short notes on (any one) 4
   • (i) Role of vitamin A in vision
   • (ii) Coenzyme form of vitamin
   • (D) Discuss in detail(any one) Working of glass electrode and jts applications Gi) Titration curve of asparate 6
3. Q5 (A) Justify any one of the following 3 marks
   • (i) acid cycle is
   • (ii) in humans is highly significant
   • (B) Elaborate on (any one) Uncouplers of ETC 3
   • (ii) Electron carriers in photosynthesis
   • (C) Explain any one of the following 3
   • (i) Application of ion exchange chromatography
   • (ii) Principle of affinity chromatography
   • (D) Attempt any one of the following 3
   • (i) Physiological role of vitamin E
   • (ii) the pH of a mixture of 0.1M acetic acid and 0.2M sodium
   • (E) Write True or False(any three) 3
   • (i) is formed at the end of phase I of glycolysis form part of gluconeogenesis, Ubiquinone is also known as CoQ10
   • (iv) Affinity chromatography relies on differences in the physical properties of the molecules to be separation of buffers
   • (v) Henderson-Haselbalch equation is used for preparation of buffers
   • (vi) Riboflavin and Lactoflavin are different biomolecules

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