BSc Biochemistry Sem V 2016 17 2016-17 Bio Chem Biochemistry IIII Advance Genetics Question Paper - Mumbai University | munotes

Questions asked in this paper

• (2) Figures to the right indicate full marks

1. Q2 (A) Fillin the blanks (any three) 3 marks
   • (a) Pribnow box consists of the following 6 nucleotide bases
   • (iii) TTAAAT
   • (b) Termination of transcription is brought about by
   • (i) sigma factor
   • (c) The specific sequence of nucleotide bases of the prokaryote mRNA that bind with rRNA of 30S ribosome is called as
   • (d) Wobble hypothesis is characterized by recognition of
   • (i) asingle codon by a single tRNA
   • (ii) more than one codon by a single {RNA
   • (iii) asingle codon by more than one t- RNA
   • (e) The total number of codon available to code for 20 amino acids
   • (i) 20 (ii) 64 (iii) 61
   • (f) The factories for protein synthesis is
2. Q2 (B)- Answer any one:
   • (a) Effect of puromycin on protein synthesis. 2
   • (b) Define:- Split gene
3. Q2 (C) Write a short note on any one 4 marks
   • (a) Characteristics of Genetic code
   • (D). Attempt any one 7 (a) Explain various post translational modifications of proteins 6
   • (b) Describe the process of transcription
4. Q3 (A) Fill in the blanks (any three):
   • (a) Selection of cosmid can be done by _ . 3
   • (i) cos site
   • (b) PBR322 is
   • (ii) bacteriopage
   • (iii) cosmid
   • (c) Vector used for cloning very large ( sequences of DNA is
   • (i) E.coli
   • (ii) phages
   • (iii) BAC
   • (i) blunt
   • (ii) sticky
   • (iii) both
   • (e) The cloning vectors containing signals for protein synthesis are
   • (i) expression vector
   • (f) DNA segment to be cloned is called as
   • (i) gene segment
5. Q3 (B) Give the role of any one enzyme : 2 marks
   • (C) Write a short note on any one 4
   • (a) Probes used for cloning (b) Restriction endonucleases
   • (D) Attempt any one 6
   • (a) Elaborate on various applications of RDT
   • (b) Write a short note on various cloning vectors 4, (A) Fill in the blanks (any three):- 3
   • (a) The technique employed for the amplification of a selected DNA
   • (ii) recombination
   • (b) cDNA contains copies of DNA sequence that are
   • (ii) tRNA
   • (c) Taq polymerase is isolated from
   • (d) Southern blotting is used for identification of
   • (ii) RNA
   • (e) During gene transfer to protect genes from nuclear digestion they are incorported in
   • (i) liposome
   • (ii) phagasome
   • (iii) phagolysosome
   • (f) Microprojectile method of gene transfer in plants involves delivery of DNA with the help of
   • (i) micromanipulator
   • (ii) bolistics
6. Q4 (B) Define any one 2 marks
7. Q4 (C) Write a short note on any one 4 marks
   • (D) Attempt any one 6
   • (a) Describe the technique used for cell free molecular cloning
   • (b) Write a short note Southern blotting
   • (A) Answer the following any one 3
   • (a) Explain theta mode of DNA replication
   • (b) Explain excision repair
   • (B) Write a short note on any one 3
   • (a) Activation of t-RNA
   • (C) Write a short note on any one 3
   • (a) YAC vector
   • (D) Explain the process of any one: 3
   • (b) Antibiotic selection
   • (E) State True or False (any three) 3
   • (a) SOS repair is an error prone repair
   • (b) POMATO is an transgenic plant
   • (g) pUC-19 is a cloning vector

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