BSc Biochemistry Sem V 2018 19 2018-19 TYBIO CHEM Biochemistry I Metabolism & Analytical Tech Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q1 A) Choose the MOST appropriate option (Any Three) 3 marks
5. Q1 The starting material for TCA is li) Glycogen is a polymer joined by glycosidic linkage ili) The substrate for Glucokinase
   • iv) Krebs cycle occurs in ‘
   • v) Glycogen breaks down into
   • vi) is a keto hexose
6. Q1 B) Define and explain any one 2 marks
7. Q1 Glycogenolysis Schematically represent any one: 4
8. Q1 Glycogenesis represent any one : 6
9. Q2 A) the MOST appropriate option (Any Three) 3 marks
10. Q1 Light reaction takes place in
    • a) grana of chloroplast. — b) thylokoids of Chloroplast c) both a and b li) First stable compound in Calvin cycle after carbon fixation is ili) Complex of ETC is not DIRECTLY involved in ATP Synthesis
    • a) I b) II c) Il
    • iv) In Complex I, the electron carrier is
    • v) is an uncoupler
    • vi) Electrons flow through ETS in reduction potential
11. Q2 B) Attempt any one: 2 marks
    • ii) Briefly explain Hill's contribution to the understanding of photosynthesis
12. Q2 C) answer any one: 4 marks
13. Q1 Describe in detail malate-aspartate shuttle li) With the help of a diagram explain the structure of ATP synthase Complex. Also state the chemiosmotic hypothesis
14. Q2 D) Answerany one in detail: 6 marks
15. Q1 Give a detailed explanation of cyclic and non-cyclic photophosphorylation
    • ii) With the help of a diagram explain ETC under the following headings: (a) Complexes involved (b) Sites of ATP generation. Add a note on Q cycle
16. Q3 A) Choose the MOST appropriate option (Any Three) 3 marks
17. Q1 The adsorbent used in TLC can be
    • a) Silica gel b) G-250 c) P-400 li) Distribution coefficient cannot be than 1.0
    • a) more b) less c) equal to ili) is the detecting agent used for seperation of sugars by paper
    • iv) is a gas used in GC Diethylaminoethyl (DEAE) ion-exchangers are exchangers
    • a) cationic b) anionic c) cationic and anionic
    • vi) The length of the column and resolution of components are related
    • a) directly b) inversely c) not
18. Q3 B) Define and explain any one 2 marks
19. Q1 Partition Coefficient
    • ii) Eluent
20. Q3 C) Write a detailed answer for any one: 4 marks
    • i) Explain the technique used to separate a mixture of sugars li) Write a note on Column Chromatography
21. Q3 D) Attempt any one: 6 marks
22. Q1 Elaborate on Ion Exchange Chromatography li) Discuss the principle and applications of Gel chromatography
23. Q4 A) Choose the MOST appropriate option (Any Three) 3 marks
24. Q1 Folic acid is important for li) is the most abundant mineral in the body ili) Lysine is _ amino acid
    • iv) The physiological of the human body is
    • v) Buffers are mixtures of
    • a) weak acids and conjugate bases
    • b) strong acids and conjugate bases
    • c) strong bases and conjugate acids) Vi) pH meter is a
25. Q4 B) Attempt any one: 2 marks
26. Q1 Mention the (a) coenzyme form and (b) biochemical role of pyridoxine li) Name any 2 electrodes used in pH meter
27. Q4 C) one in detail: 6 marks
28. Q1 Discuss in detail the chemistry of Vitamin A and its role in vision li) Derive the Henderson Hasselbalch equation
29. Q4 D) in detail any one : 8 marks
30. Q1 With the help graph, explain the titration curve of a basic amino acid. Mention the pKa and the pI values With a special mention of the hormones involved, explain the mechanism of absorption and mobilization of calcium in the human body
31. Q5 Answer in brief any fourof the following: 12 marks
32. Q1 Write a short note on: Gluconeogenesis
33. Q1 Elaborate on the pyruvate dehydrogense enzyme complex il) Write an elaborate note on Proton motive force ili) Give the principles of GLC and Affinity Chromatography Explain the different types of chromatographic techniques
    • iv) Compare and contrast: Water soluble and fat soluble vitamins
    • iv) Draw the titration curve of a neutral amino acid, and mention pKa values and pl
34. Q5 B) State TRUE or FALSE: (any four) 3 marks
    • ii) Lysine has two ionizable groups ili) TCA is also called Citric acid pathway
    • iv) Anabolism involves expenditure of ATP
    • v) Only sugar can be seperated by paper chromatography

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