BSc Biochemistry Sem V 2018 19 2018-19 TYBIO CHEM Biochemistry III Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks the blanks: (any three) 3
   • i) The leading strand of DNA is synthesized in direction ili) In light thymine dimers may be formed
   • iv) undergoes deamination, and becomes Uracil
4. Q1 B) and explain any one of the following: 3 marks
5. Q1 DNA repair
6. Q1 C) Write a detailed note on any one: 6 marks
7. Q1 Excision repair li) Proteins involved in replication any one of the following: 8
8. Q1 In detail discuss the mechanism of replication
   • ii) Elaborate on direct repair and mismatch repair
9. Q2 A) in the blanks: (any three) 3 marks
10. Q1 Rifampicin is an inhibitor of li) is present in DNA but not in RNA ili) With mRNA codon, the tRNA with 5'GAG3' will
    • iv) Transcription takes place in the
11. Q2 B) and explain any one of the following: 3 marks
    • i) Promoter
12. Q2 C). Write a detailed note on any one : 6 marks
    • i) Activation of tRNA li) Capping and tailing of RNA
13. Q2 D) any one of the following: 8 marks
    • i) Describe the post translational modifications transcription in li) Write short notes on : a) RNA Polymerase (ii) Initiation of
14. Q3 A) in the blanks: (Any three) Klenow fragment is obtained by proteolytic cleavage of 3 marks
    • ii) In the restiction endonuclease EcoRI, "R" stands for enzyme is used to seal the nick in DNA
    • iv) The vector used to study protein expression is a vector
15. Q3 B) and explain, any one: 3 marks
    • i) Palindrome
16. Q3 C) Answer any one of the following: 6 marks
    • i) State true or false giving detailed reasons: “Shuttle vectors are superior to conventional vectors.”
17. Q11 Give the role and mechanism of action of any two enzymes involved in RDT
18. Q3 D) Answer any one: 8 marks
    • i) “RDT has transformed the field of medicine and agriculture.” Explain the statement giving suitable examples
    • ii) Elaborate on of plasmids and bacteriophages that makes them ideal cloning vectors?
19. Q4 A) in the blanks: (Any three) 3 marks
20. Q1 DNA is injected directly into cells by
    • ii) of nitrocellulose at high temperature is known as Transfer of recombinant plasmid into E.coli cells needs
    • iv) The process of introduction of foreign DNA into an animal cells is called
21. Q4 B) Define explain any the following: 3 marks
22. Q4 C) Attempt any one of the following: 1). Discuss any two methods of recombinant gene transfer into 6 marks
    • ii) Write a note on the contribution of E.M. Southern in the field of RDT
23. Q4 D) any one: 8 marks
    • ii) Compare and contrast: Gene library and cDNA library
24. Q5 Answer any four of the following: 16 marks
    • i) Explain the different modes of DNA replication
    • i) Describe SOS repair mechanism li) Write a note on split genes
    • ii) Explain the process of initiation of translation ili) Give a comparative account of cloning and expression
    • iii) In detail, explain the role of ligase in RDT
    • iv) Justify: 'Liposomes are ideal vectors for introduction of
    • iv) Elaborate on the technique of colony hybridization
25. Q5 B) State TRUE or FALSE: (any four) 4 marks
26. Q1 Restriction enzymes act only as exonucleases Replication starts at origin of replication
    • iii) Plasmids are always smaller than bacteriophages
    • iv) All Polymerase are present in the cytoplasm Introduction of genes using a virus is termed as
    • v) Transformation
    • vi) Rho factor is involved in initiation of Replication

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