BSc Biochemistry Sem V 2017 18 2017-18 Bio Chem Metabiolosm & Ana. Tech Question Paper - Mumbai University | munotes

Questions asked in this paper

• (2) Figures to the right indicate Full Marks

1. Q1 (A) Choose the MOST APPROPRIATE answer (Any Three) : 3 marks
   • (i) mammalian cell does not have aerobic pathway of glucose
   • (ii) In anaerobic glycolysis does not stop at pyruvate but forms lactate, because pyruvate is toxic in larger concentration
   • (b) pyruvate can form glucose back
   • (c) Because this allows the regeneration of NAD from NADH, (ili) CO, is not produced in the reaction catalysed by the enzyme
   • (a) Pyruvate dehydrogenase
   • (b) Succinate dehydrogenase
   • (c) Isocitrate dehydrogenase
   • (iv) Which statement is not true of HMP shunt pathway?
   • (b) NADPH is produced
   • (c) Pentoses are produced
   • (v) Neoglucogenesis occurs predominantly from the following compounds
   • (vi) ATP molecules are produced on complete oxidation of acetyl CoA in the citric acid cycle
   • (B) Define and explain (any one) : 2
   • (i) Catabolism
   • (ii) Transketolase
   • Q.P. Code : 25027
   • (C) Write short notes on (any one) : 4
   • (i) Multienzyme complex
   • (D) Attemtp (any one) : 6 (ui) Compare : Glycogenolysis and Glycogenesis
2. Q2 (A) Choose the MOST APPROPRIATE answer (Any Three) : 3 marks
   • (ii) During the dark reaction of photosynthesis
   • (a) Water is split
   • (b) CO, is reduced to organic compounds
   • (c) Chlorophyll is activated Chlorophyll-a differs from chlorophyll-b in having a
   • (iv) Which one of the following statements best describes the enthalpy change
   • (a) The energy consumed when chemical bonds are broken during a
   • (b) The difference between the energy released by bond formation and the energy consumed by bond cleavage during a chemical reaction
   • (c) The increase in disorder of the system as a reaction proceeds
   • (v) Glycerol phosphate accounts for generation of ATP
   • (B) Define and explain (any one) : 2
   • (i) Antenna molecules
   • (ii) DNP
   • Q.P. Code : 25027
   • (C) Answer the following (any one): 4
   • (i) Describe the significance of Complex HI to ETC
   • (i) Schematically explain cyclic photophosphorylation
   • (D) Answer the following (any one) : 6
   • (i) With the help of a diagram, explain the structure of the ATP synthase complex and its contribution to the generation of a proton motive
   • (ii) Elaborate on C3 cycle with special emphasis on the role of rubisco Explain the stoichemetry of the C3 cycle
3. Q3 (A) Choose the MOST APPROPRIATE answer (any three) : 3 marks
   • (i) Ion exchange chromatography is based on
   • (a) electrostatic attraction
   • (b) electrical mobility of ionic species
   • (c) adsorption chromatography
   • (ii) The right expression for the appearance of a solute in an effluent is (where is the elution volume of a substance , V, void volume, kD distribution constant and Vi internal water volume)
   • (iii) A student sets up a paper chromatogram and places a spot of green food dye on the origin. After six minutes the solvent has moved 12 cm anda blue spot has advanced 9 cm. After fourteen minutes the solvent has advanced a further 4 cm. How many cm from the origin is the blue spot likely to be?
   • (iv) is the most suitable gas to use as a carrier gas in a gas chromatogram
   • (v) In chromatography, the mobile phase can be
   • (a) Solid or liquid (b) Liquid or gas (c) Gas only
   • Q.P. Code : 25027
   • (vi) types of chromatography involves the process, where mobile phase moves through the stationary phase by the influence of gravity or capillary
   • (a) Column Chromatography
   • (c) Planar Chromatography
   • (B) Define and explain (any one): 2
   • (i) Gradient elution
   • (C) Write short note on (any one) :
   • (i) Column chromatography
   • (ii) Principle and applications of gel permeation chromatography
   • (D) Answer the following (any one): 6 A mixture of glucose, xylulose and lactose has to be resolved into separate components. Suggest a technique and explain its principle as well as the procedure for the same
   • (ii) Components with isoelectric pH as 2.4, 5.9 and 9.8, need to be separated from a plant extract. (a) Give the principle of chromatographic technique suitable for this separation. (b) Discuss the technique in detail starting with the stationary phase, mobile phase and the order of elution
4. Q4 (A) Choose the MOST APPROPRIATE answer (any three) : 3 marks
   • (i) Which one of the following cofactors is correctly matched with the vitamins itis derived from? form of Vitamin A is required for vision?
   • (a) All cis retinal (b) All trans retinal (c) 11 cis-retinal
   • (a) secretion of enzymes
   • (b) keratinisation of epidermal cells
   • (c) absorption of harmful enzymes
   • Q.P. Code : 25027
   • (iv) Ascorbic acid acts as a
   • (a) oxidizing agent
   • (b) reducing agent
   • (c) both reducing and oxidizing agent
   • (v) The equilibrium constant of ionisation reaction of pure water is
   • (vi) At zwitterionic form, an amino acid will act as a
   • (a) proton donor
   • (b) proton acceptor
   • (c) both proton donor and acceptor
   • (B) Define and explain (any one) : 2
   • (i) Titration curve
   • (ii) Niacin
   • (C) Answer the following (any one): 4
   • (i) the blood calcium level maintained in the body? Emphasize on the role of PTH
   • (ii) Derive the Henderson Hasselbalch equation. Prepare a buffer solution that has a pH of 5.27. If there are 10mmols of acetic acid, how many mmols of sodium-acetate will be needed? (pK, of acetate buffer is
   • (D) Answer the following (any one): 6
   • (i) Define buffering capacity. With ionisation forms and pKa values, explain the titration curve of a basic amino acid
   • (ii) Discuss in detail the role of transducin in the Wald’s Visual cycle
5. Q5 (A) Attempt any one of the following : 3 marks
   • (i) Schematically represent the oxidative phase of HMP
   • (ii) Give the significance and characteristics of the glyoxalate cycle
   • Q.P. Code : 25027
   • (B) Answer the following (any one): 3
   • (i) Give the redox potential NADH Coenzyme Q
   • (ii) Compare and contrast : Oxidative and photo-phosphorylation
   • (C) Answer the following (any one): 3
   • (i) Give the principle and applications of gas chromatography
   • (ii) For chromatography, state the role of : (a) Dextran blue, (b) Silica gel
   • (D) Answer the following (any one) : 3
   • (ii) Briefly explain the working and principle ofa pH meter
   • (E) State True or False (any three) : 3
   • (ii) PDH complex has lipoic acid as a cofactor (iit) Cytochrome c has higher redox potential to that of hydrogen
   • (iv) Above the pI, an amino acid is positively charged
   • (v) Water soluable vitamin deficiencies generally do not occur
   • (vi) The synonym of Glyoxalate cycle is HMP pathway

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