BSc Biochemistry Sem V 2018 19 2018-19 TYBIO CHEM Biochemistry III Advanced Genetics & RDS Question Paper - Mumbai University | munotes

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1. Q2 Choice is internal
2. Q4 Non-programmable calculators are permitted
3. Q5 Figures to the right indicate full marks
4. Q1 A) Choose the MOST APPROPRIATE answer (any three): 3 marks
   • i) DNA replication in vivo is discontinuous due to
   • a) sister chromatid exchange b) being restricted to synthesis in the 5’ to 3' direction
   • c) topoisomerases cutting the DNA in a random fashion
   • ii) enzyme removes the RNA primer during replication
   • iii) Suppose a mutation occurs in a cell such that normal Okazaki fragments were created during DNA replication but were not linked together into a continuous strand. The gene for which enzyme would you predict was altered by this mutation? and new strands of DNA in bacteria can be distinguished by
   • v) Thymine dimers may be repaired by in humans
   • vi) Cytosine undergoes deamination, and becomes
   • B) Answer in brief any one: 02 mode of replication of DNA
   • C) Write a short note on any one: 04
   • D). Answer any one of the following: 06 on the mechanism of replication in E.coli
   • ii) Write brief note on: (a) SOS repair (b) Photoreactivation
5. Q2 A) Choose the MOST APPROPRIATE answer any three: 3 marks
   • i) In which of the following actions does RNA polymerase differ from DNA polymerase?
   • ii) As a ribosome translocates along a mRNA molecule by one codon occurs
   • a) the tRNA that was in the A site moves into the P site
   • b) the tRNA that was in the P site moves into the A site
   • c) the tRNA that was in the A site moves to the E site and is released
   • iii) Which of the following statement is true about protein synthesis in prokaryotes?
   • a) Extensive RNA processing is required before prokaryotic transcripts can be translated
   • b) Translation can begin while transcription is still in progress
   • c) Unlike eukaryotes, prokaryotes require no initiation or elongation factors
   • iv) is not directly involved in translation
   • v) nucleotide base is not found in RNA
   • a) Guanine (c) Uracil
   • vi) The tRNA with 5’AUC3’ will form a codon- anticodon base pairing interaction With
   • B) Answer in brief, any one of the following: 02 on the action of Puromycin
   • ii)Explain : Sense strand in transcription
   • C) Write short notes on any one of the following: 04
   • D) Answer any one of the following: 06
   • i) Discuss the mechanism of RNA formation in prokaryotes
   • ii) Explain in detail initiation and termination phase of protein synthesis
6. Q3 A Choose the MOST APPROPRIATE answer any three: 3 marks
   • i) is derived from proteolytic cleavage of DNA pol I
   • ii) A technique that measures degree of genetic similarity between pools of DNA sequences is
   • iii) In restriction endonuclease EcoRI, "co" stands for cleaves DNA at
   • v) When two DNA pieces cut with the same restriction enzyme are combined, sticky ends
   • a) anneal by covalent bonds b) anneal by DNA ligase
   • c) anneal by complementary base pairing and hydrogen bonds
   • vi) Which arrangement of the following four enzymes represents the order in which they would be used in a typical gene-cloning experiment resulting in the insertion of a cDNA bacterial plasmid? Begin with the gene's mRNA transcript
   • B) Define and explain any one of the following in brief: 02
   • C) Answer any one of the following: 04
   • i) Describe use of RDT for pest resistant crops and for therapy of diabetes
   • ii) Ifa gene needs to be isolated from a source and has to be inserted into another gene, discuss the enzymes employed for the same Answer any one: 06 “Shuttle vectors are more efficient as compared to cloning vectors.”
   • ii) Justify in detail: “Vectors are one of the most essential tools for RDT’
7. Q4 A) Choose the MOST APPROPRIATE answer (any three): ampicillin-sensitive culture of E. coli is transformed with a plasmid that contains the gene of interest plus an ampicillin-resistance gene. If it is then plated on an ampicillin-containing 3 marks
   • a) it it will form white colonies it will not form any
   • ii) Virus mediated gene transfer is known as
   • iii) Chemical used in transformation is
   • a) PEG b) c) MgC12
   • iv) Introduction of DNA using a hypodermic needle is known as
   • v) are used for introduction of genes in plants
   • vi) mutants are used in Lac selection
   • B) Define and explain any one of the following: 02
   • C) Attempt any one of the following: 04
   • i) Discuss the technique and advantages of gene transfer that involves the use of electric
   • ii) Give E.M. Southern's contribution to the field of RDT"
   • D) Answer any one: 06
   • i) Elaborate cell-free molecular cloning
   • ii) Mahesh has inserted the gene of interest in a plasmid in the tetracycline resistant gene, Discuss the method for selection of the transformant
8. Q5 A) note on any one of the following: 3 marks
   • i) Write a note on DNA Polymerase I
   • ii) Justify: "A single dimer of Polymerase III can simultaneously conduct replication on both leading and lagging strands"
   • B). Answer in brief any one the following: 03
   • i) Charging of tRNA
   • ii) Give the significance of rho factor and sigma factor
   • C) Answer in brief any one the following: 03
   • i) Discuss the different techniques of probe labelling
   • ii) Depict the structure of pBR322, with a neat-labelled diagram ONLY
   • D) Answer in brief any one: 03
   • i) Elaborate on the different types of genomic libraries
   • ii) Discuss the use of liposomes for gene transfer
   • Q.P. Code :20218
   • E) State true or False: (any three) 03
   • i) Replication is not dispersive in prokaryotes
   • ii) Cystine dimers form in UV light
   • iii) Polycistronic RNA are only observed in prokaryotes
   • iv) Protein synthesis takes place in the the cytosol
   • v) pUC 18 is only a cloning vector

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