BSc Biochemistry Sem V 2016 17 2016-17 BIOCHEMISTRY PAPER 3 ADVANCED GENETIC & RD 5 Question Paper - Mumbai University | munotes

Questions asked in this paper

• (2) Figures to the right indicate full marks,

1. Q2 (A) Choose the MOST appropriate option (Any Three)
   • (i) What is true regarding RNA processing?
   • (a) Involves removal of exons
   • (b) Involves removal of one or more introns
   • (c) Occurs in prokaryotes
   • (ii) Genetic code is degenerate, means
   • (a) RNA is rapidly degraded
   • (b) code is not organisms
   • (c) some amino more than one codon
   • (iii) strand of DNA has similarity with RNA nucleotide except for between T and U
   • (iv) Alternate splicing a characteristic feature of
   • (a) (b) yeast (c) humans
   • (v) sequence is present on (viJoRecycling of EF- Tu, involved in translation requires
2. Q2 in brief any one: 2 marks
   • (i) Which process of the Central Dogma does Rifampicin and Puromycin affect and how?
   • (ii) Define: Polycistronic Write short notes on > 9, (C) INY One of the F
   • (i) Charging of (ii) 2 (D) Answer in detail ANY two: a
   • (i) Elaborate on mechanis ‘
   • (ii) Discuss m of transcription, av modifications in detail
   • (A) the MOST Option (Any Three):
   • (a) Esterase S to split of sugar phosphate bonds
   • (b) enzyme 'tansferases catalyses addition of nucleotides to Bree (a) 3 terminus of DNA
   • (b) terminus of
   • (c) terminus of
   • (iv) Type cut at a site that differs, and is
   • (a) cloning
   • (v) Transcriptase is used for synthesis of RNA from protein
   • (B) in brief any one: 2
   • (i) Enlist two characteristics of an ideal plasmid
   • (ii) Define: Cosmid
   • (C) Answer any one of the Write note on: Probes
   • (D) Attempt any one:
   • (i) A variety of ire with on the above \
3. Q4 (A) Choose the MOST appropriate option: (Any 4 marks
   • (i) Which of the following would be be
   • (a) Genomic made from mouse and kidney cells may
   • (b) cDNA. libraries made from mouse liver and kidney
   • (c) Genomic and cDNA libraries, made from mouse liver colls
   • (ii) CaCl,
   • (a) transformation
   • (c) microinjection
   • (iii) Transformed cells of X-gal coloured
   • (v) gun technique helps to overcome the problem of Complex structure of animal cell
   • (b) cell wall of plant cell
   • (c) peptidoglycan cell wall of bacteria is often used to describe virus-mediated gene transfer into
   • (a) Transformation
   • (c) Transduction
   • (B) Define the terms any one:
   • (C) Write short notes on any one:
   • (i) Use of antibiotics for selecti
   • (ii) Gene election and screening
   • (D) Elaborate on any one: ion for effective results. a ure of carrying out the same
   • (ii) iscuss the technique of Southem blotting 5 (A) Answer any one:
   • (i) Why is SOS repair error prone?
   • (ii) Justify: Replication is
   • (B) Write brief notes on any one: 3
   • (i) Modifications of tRNA
   • (11) Termination of translation
   • (C) Attempt any one: What is role of reverse in recombinant DNA (il) Write a note on BACY Write a brief note 3
   • (E) State true or false (Any Three): 3
   • (i) YAG@ exhibits the lytic and the lysogenic phases in their life cycle
   • (ii) fork is bidirectional in nature “The C end of a protein is synthesized first followed by the N end Sigma factor is in termination of translation

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