BSc Biochemistry Sem V 2019 20 Oct 2019-20 BIO CHEM BIOCHEMISTRY PAPER III 14.10.19 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q1 the blanks: 4 marks
   • i) The site for DNA replication is
   • ii) Thymine dimers occur due to light The enzyme used to join bits of DNA is
   • iv) Okazaki fragments are present on strand
5. Q1 B) Write a note on (any one): Answer the following: (any two) 12 4 marks
   • i) Discuss the various proteins required to be synthesised in a cell to enable it to
   • ii) Elaborate on the Excision repair mechanism iil) Discuss the structural mutations that can occur during or after replication
6. Q2 A) Fill in the blanks: 4 marks
   • i) Post-transcriptional modification occurs in
   • ii) 1s codon iil) The synthesis of polynucleotide chain of mRNA is catalysed by the enzyme
   • iv) Protein synthesis in bacteria takes place on/in
7. Q2 B) Write a note on (any one): 4 marks
   • i) code
   • ii) Splicing Answer the following: (any two) 12
   • i) Write a note on (1) inhibitors of mechanisms of central dogma of molecular biology
   • ii) Schematically ONLY represent the process of elongation in translation Explain the process of initiation of protein synthesis
8. Q3 A) Fill in the blanks: 4 marks
   • i) cleaves DNA at
   • ii) In pUC vectors, if the gene of interest is inserted in gene iil) Restriction endonucleases isolated from unidentified micro-organisms is named as
   • iv) chemical is used to increase the copy number of a plasmid
9. Q3 B) Attempt the following: (any one) 4 marks
   • i) Diagrammatically represent and explain the process of cloning a foreign gene into a
   • ii) Create a note on genetically modified food
10. Q3 C) Answer the following: (any two) 12 marks
    • i) Write a short note on probe. Briefly explain about labelling of probe and its
    • ii) Elaborate on ‘Molecular Scissors’ and ‘Molecular
    • iii) Justify: is an ideal vector’
11. Q4 A) Fill in the blanks: 4 marks
    • i) At temperature denaturation of DNA double helix takes place in PCR
    • iv) developed the polymerase chain reaction
12. Q4 B) Attempt the following: (any one) 4 marks
    • i) Highlight on the contribution of Southern to the field of recombinant DNA
    • ii) Discuss the (a) experimental set-up and (b) advantages of a chemical method of gene
13. Q4 C) Answer the following: (any two) 12 marks
    • i) Explain selection of recombinant cells by the use of antibiotic-resistance gene
    • ii) Elaborate on a technique of DNA amplification a stepwise manner, explain the formation of a DNA library. Also, state the difference between a cDNA library and genomic library
14. Q5 A) Define and explain: 8 marks
    • a) Okazaki Fragments
    • b) Aneuploidy
    • e) J unit of restriction endonuclease
    • g) Colony hybridization
    • h) Transformation
15. Q5 B) State True or False with justification: iil) Lac selection method is also known as blue-white colony method 12 marks
    • iv) All repair mechanisms are error free Meselson and Stahl proved that translation is by semi-conservative process
    • vi) SSB are a requirement in repair

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