BSc Biotechnology Sem V 2016 17 2016-17 BIOTECHNOLOGY PAPER 3 PRINCIPLES GENE CLONING & RECOMBINANT DNA TECH Question Paper - Mumbai University | munotes

Questions asked in this paper

• (1) Attempt all questions,

1. Q3 (a) directed (any three)
   • (i) What is sequenase?
   • (ii) Define : annealing
   • (iii) Give the role of primers
   • (iv) State one application of DNA typing
   • (v) Explain: minisatellite sequence
   • (vi) Fill in the blank: Restriction enzyme generated are
   • (b) Give an account of the following ‘- (any two) <)
   • (i) Screening of genomic libraries PCR and its applications
   • (iv) Any one method used for DNA: Q
2. Q4 (a) Give the significance of (any
   • (v) Vaccinia virus
   • (b) Answer the following:- (any 12
   • (i) Discuss the advanta a live recombinant vaccine over heat
   • (ii) How would you clop a vaccine against VY cholerae?
   • (iii) Describe the of action and application of peptide vaccines
   • (iv) Comment ex-vivo and in-vivo approaches to targeting
3. Q5 Write short notes three) 15 marks
   • i) of sickle cell anemia using RFLP
   • (ii) restriction and modification of restriction enzymes of cDNA library Mode of action and applications of

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