BSc Biotechnology Sem V ATKT BIOTECH BIOTECHNOLOGY PAPER III GENETICS & MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory
2. Q2 All questions carry equal marks
3. Q1 a. Explain the terms (Any three) 3 marks
   • b. Explain the following ( Any two) 12
4. Q1 Types of bacterial transformation giving suitable examples
5. Q2 Genetic mapping during conjugation in E. coli
6. Q3 Production of F’ factor
7. Q4 Test for determining the units of function in region
8. Q2 a. Doas directed (Any three) 1 What is an operon? 2 Give an example of non-composite transposon 3 Name the enzyme synthesized by lac Y gene 4 Give an example of an IS element in E.coli 5 What is an aporepressor? 6 State true or false: - The promoter and operator regions are upstream from the trpE gene 3 marks
   • b. Discuss the following (Any two) 12
9. Q1 Catabolite repression
10. Q2 Lysogenic development in phage
11. Q4 Composite and non-composite transposons in prokaryotes Answer the following (Any three) 03 1 What is an “expression vector’? 2 State the significance of “sequenase’ 3 What are “YACs’? 4 Give significance of opine 5 What is “multiple cloning site’? 6 State one application of DNA kinase
    • b. Attempt the following (Any two) 12
12. Q1 Discuss pBR322 vector
13. Q2 What are restriction enzymes? Explain its types
14. Q3 Explain the use of ‘binary vector system’ using a neat labelled diagram
15. Q4 Give a detailed account of ‘DNA ligases’
16. Q4 a. Define the following (Any three) 3 marks
    • b. Give an account of the following. (Any two) 12
17. Q1 Describe Random - primer labeling
18. Q2 Explain restriction mapping
19. Q3 How would you cDNA library?
20. Q4 State how genes can be identified in libraries by complementation of
21. Q5 short notes on (Any three) 15 marks
    • b. transduction
    • c. Screening of genomic library
    • e. Terminal transferase

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