BSc Biotechnology Sem V 2016 17 2016-17 BIOTECHNOLOGY PAPER 3 Genetics & Molecular Biology Question Paper - Mumbai University | munotes

Questions asked in this paper

• N.B. Attempt all questions j

1. Q1 (a) Explain the following terms (Any three)
   • (i) Minimal media 3
   • (b) Attempt the following (Any two)
   • (i) Genetic mapping can be carried out using transformation. Justify
   • i) explain generalised transduction Elaborate on types of phage mutafits
   • (iv) Comment on the circularity of the E.coli map 9, (a) State the significance of (Any
   • (iv) Operator
   • (v) Lac [5 mutaties!
   • (b) Describe the following (Any two) Repressible operons with an example Cis\dominant mutations with suitable examples
   • (iii) transcription events in phage X Integration of IS element into chromosomal DNA
2. Q3 (a) Bo ‘as directed (Any th ree) 3 marks
   • (i). What is a cosmid ? XV (ii) - Define Shuttle vectors in the plank both strands of DN When make cults a at the sae position, they result in State the of gene in yeast episomal plasmics Give one that regult in star activity in restriction of the following (Any two) L
   • (iii) DNA polymerases and its types
   • (iv) DNA Ligases and their applications 4 (a) the following (Any three) ‘
   • (i) Chromosomal libraries
   • (v) site linker
   • (vi) Autoradiogram
   • (b) the following (Any two) How would you a DNA library ?
   • (ii) State how genes identified in libraries by complementation of
   • (iii) Describe Randdm - primer labeling 3, Write short notes.on the following (Any three) (iil of genomic librar ! Positive control of the lac operon Distinguishing features of composite and non-composite transposons anned by CamScanner

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