B.Sc. (Biotechnology) Sem V 2022 2023 Nov 2023 GENOMES AND MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Select the correct alternative (Any six) 6 marks
4. Q1 species of Agrobacterium cause crown gall disease
5. Q2 bombardment is otherwise called as
6. Q3 was the first plant developed using protoplast fusion Which part of lipids forms the wall of transient aqueous pores after electroporation ?
7. Q5 an indirect gene transfer method from the following given method
8. Q6 stands for
9. Q7 of the following gene can be used for the improvement of seed protein quality
   • a. EPSPS protein gene from petunia b. zein proteins genes from maize
   • c. cry protein genes from BT d. CHS protein gene from petunia
10. Q8 Ina Binary vector system, a small intermediate vector system is used along with a selectable marker, it is called
11. Q9 Name the gas used for particle propulsion in biolistic transformation?
12. Q1 b. Answer the following questions: (Any Two) 14 marks
13. Q1 Describe the need for seed quality protein improvement and the role of transgenic technology with suitable examples
14. Q2 Explain different vectors used for plant cells
15. Q3 Explain the electroporation and microprojectile bombardment mechanism of gene
16. Q2 a. Select the correct alternative (Any six) 6 marks
17. Q1 transfection in the embryonic stem cells method, DNA integrated at the non
    • a) b) c) correct position
18. Q2 Transgenic fish is used as biosensors of environmental pollutants
    • a) medaka b) d) salmon
19. Q3 the full form for YACs vectors for animal cells
20. Q4 element within the is deleted to prevent the production of vector RNA from a promoter
21. Q5 One of the aims of targeted gene disruption (gene knockout) is to determine the development and physiological consequences of a particular gene
    • a) inactivation b) transcription d)
22. Q6 In the bacteriophage genome, A lox P site consists of two 13-base-pair that are separated from each other by an 8-bp spacer sequence
23. Q7 In the lentiviral transfer method, PPT stand for?
24. Q8 DNA is microinjected into pronucleus of a fertilized egg
25. Q9 cloning livestock by nuclear transfer method, induced somatic cells are fused to an enucleated oocyte with
    • a). short-duration electric pulses
    • b) electric pulses
    • c) short-duration high electric pulses
26. Q2 b. Give an account on the following questions: (Any Two) 14 marks
27. Q1 Retroviral method for production of transgenic mice
28. Q3 Transgenic sheep and its application Select the correct alternative (Any six) 06 The media used to isolate host cells containing pUC vector is
29. Q2 will happen when M 13 infects the host cell?
    • a. Host cell is lysed
    • b. Host cell continues growth at same rate
    • c. Host cell growth is arrested after a few generations
    • d. Host cell growth is slowed down and viral particles are released
30. Q3 vector containing regulatory sequences is called The T7 RNA pol gene is introduced in the
    • c. Helper plasmid d. Any of them
31. Q5 translation, the commonly used amino acid is
    • a. b. Tryptophan c. Methionine. d
32. Q6 The problem of repetitive DNA being a part of the probe and creating a problem while identifying the overlapping fragment is severe in
33. Q7 To increase the concentration of mRNAs in the cell extract, use a column lined with
34. Q8 Identification of a specific protein in a complex mixture of proteins is done by
35. Q9 used for in vitro synthesis of CDNA from mRNA
    • a. b. Klenow fragment
36. Q3 b. Discuss the following: (Any Two) 14 marks
37. Q1 Southern Blotting
38. Q2 Method to screen a genomic library
39. Q3 YAC as a cloning vector
40. Q4 a. Select the correct alternative (Any six) 6 marks
41. Q1 ddNTPs are used in sequencing DNA because
    • a. ddNTPs are fluorescent
    • b. are incorporated very efficiently into DNA
    • d. prevent further DNA synthesis once they are incorporated into the
42. Q2 derives its name from the fact that
    • a. The bases are detected by pyrolysis
    • b. It uses the enzyme apyrase to detect the bases
    • c. It detects pyrophosphate released during base incorporation
    • d. generates pyrograms as output
43. Q3 Double-stranded RNA is cleaved by a nuclease called as Dicer and small fragments are generated known as
    • a. Short interfering RNAs b. Long interfering RNAs
    • c. Short interspersed RNAs d. Long interspersed RNAs
44. Q4 short strands of double-stranded DNA which have one blunt end and one staggered end are called The role of apyrase enzyme in pyrosequencing 1s
    • b. To detect nucleotide
    • c. primer
    • d. nucleotide
45. Q6 are proteins secreted by
46. Q7 enzyme that creates a double-strand break at the target site for genome editing is
47. Q8 The PAM sequence serves as Binding signal for Cas9 b. Promoter sequence
48. Q9 DNA sequencing method developed by Sanger and Colleagues in 1977 is
49. Q4 b. Give an account of the following questions: (Any Two) 14 marks
50. Q1 Pyrosequencing as a method for DNA sequencing
51. Q2 CRISPR- Cas for gene editing
52. Q3 How does HGP help in the diagnosis and treatment of genetic diseases? Write Short notes on the following (Any four) 20
    • b. Vir genes
    • d. Radioactive labeling of DNA
    • e. Expression vector
    • f. Compare Sanger’s enzymatic method and Maxam-Gilbert chemical method of

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