BSc Biotechnology Sem V 2018 2019 2019 Biotech III Genetics & Molecular Biology Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory
2. Q2 All questions carry equal marks
3. Q1 a. Explain the terms (any three) 3 marks
   • b. Explain the following ( Any two) 12
4. Q1 Types of phage mutants
5. Q3 Recombinational analysis of rll mutants
6. Q4 Mapping of genes by Interrupted mating
7. Q2 a. Doas directed (Any three) 1 What is an Inducer? 2 Give an example of composite transposon 3 Name the enzyme synthesized by lac Z gene 4 Fill in the blank:- IS elements were first identified in 5 Name the product of trpR gene 6 State true or false: - The leader region is the short region between the promoter—operator region and trpE 3 marks
   • b. Discuss the following (Any two) 12
8. Q1 Positive control of the lac operon
9. Q2 Induction of lytic pathway in A phage
10. Q3 Regulatory mutations in lacl gene Answer the following (Any three) 03 1 State the significance of BAC vectors 2 What are shuttle vectors? 3 State the importance of phagemid vectors 4 Name the enzyme which uses DNA as template to synthesize DNA 5 Give significance of enzyme DNA ligase 6 State an application of alkaline phosphatase
    • b. Attempt the following (Any two) 03
11. Q1 Justify: Using pBR322 vector has some advantages as well as
12. Q2 Explain the use of ‘multiple cloning site’ and ‘blue white selection’ in
13. Q3 Elaborate on the use of Polynucleotide kinase
14. Q4 Give a detailed account of ‘reverse transcriptase’ with respect to its sources and applications
15. Q4 a. State the significance of the following. (Any three) 3 marks
    • b. Give an account of the following. (Any two) 12
16. Q1 Analysis of cloned DNA
17. Q2 Construction of genomic DNA Library
18. Q3 Nick Translation in Probe synthesis
19. Q4 Synthesis of cDNA
20. Q5 Write short notes on (Any three) 15 marks
    • a. Complementation test
    • b. Specialized transduction
    • d. Organization of Ti plasmid
    • e. Types of restriction enzymes
    • f. IS elements- Discovery and Properties

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