BSc Biotechnology Sem V 2016 17 2016-17 BIOTECHNOLOGY PAPER 4 INDUSTRIAL BIOTECHNOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q3 (a) State the significance of (any three)
   • (i) Hops
   • (ii) Area/volume ratio in surface fermentation
   • (iii) SO, in wine making
   • (v) Semisynthetic Penicillin
   • (b) Discuss (any two) 12
   • (i) The process of white wine production
   • (ii) Malting, mashing and kettling in beer making
   • (iv) Production of any one single cell protein
2. Q4 (a) Explain the terms (any three) 3 marks
   • (v) Reverse osmosis (vi)
   • (b) Elaborate on (any v 12
   • (i) Foam separation and filtration in DSP
   • (ii) Any two types of batch filters
   • (iii) Cell disruption by abrasive, freeze thaw and ultrasonication (iv)
3. Q5 Write short notes on (Any three) 15 marks
   • (i) jet fermenter pH measurement in a fermenter
   • (iv) Criteria for inoculum transfer in a fermenter
   • (v) Extraction of penicillin
   • (vi) Precipitation in DSP
   • N.B. (1) All questions are compulsory
   • (2) All questions carry equal marks
4. Q1 (a) Do as directed (Any three) fe 3 marks
   • (i) Define "Aspect ratio" with respect to
   • (ii) Name the gauge used to measure pressure in
   • (ii) State a method used to determine
   • (iv) What is the significance of inlet-exit gas analysis in a State the function of riser tube in an
   • (vi) What is the role of feed port ina
   • (b) Answer the following (Any two) 1
   • (i) Explain how foam is sensed in a fermenter, Elaborate on the working and application of with an inner loop
   • (iii) What is the significance of dissolved oxygen measurement in fermenter? How is it
   • (iv) Discuss bioreactors used for Animal cell culture
5. Q2 (a) Define (Any three) 3 marks
   • (v) respect to inoculum development
   • (b) the following (Any two) Justify: Secondary screening yields information needed to evaluate true potential of a microorganism for industrial usage
   • (ii) Discuss the scale down methods With a suitable example explain inoculum development process A (iv) Elaborate on crowded plate technique and its limitations

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