BSc Biotechnology Sem V ATKT BIOTECH GENOMES & MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Explain the terms: (Any six) 6 marks
4. Q1 plants
5. Q6 Co-cultivation
6. Q8 Wound response
7. Q9 Selectable marker Answer the following questions: (Any two) 14
8. Q1 Describe liposome mediated gene transfer
9. Q2 Add a note on any one pTi derived vector system
10. Q3 Explain the role of viruses in plant transgenesis
11. Q2 a. Explain the following (Any six) 6 marks
12. Q2 founder
13. Q3 Karyogamy
14. Q6 Quiescent state
15. Q7 gene
16. Q2 b. Give an account of the following questions: (Any two) 14 marks
17. Q1 Establishment of transgenic mouse by DNA microinjection
18. Q2 Positive-negative selection system for transgenic mice
19. Q3 Applications of transgenic fish
20. Q3 a. Doas directed: (Any six) 6 marks
21. Q1 significance of cosmid vectors
22. Q2 State the significance of ‘multiple cloning site’ in modern vectors
23. Q3 significance of ‘shuttle vectors’
24. Q5 chromosomal library
25. Q6 the significance of ‘Southern blotting’
26. Q7 Name the membrane used for transfer of DNA from gel during
27. Q8 What is vector’?
28. Q9 vector that employs ‘blue white selection strategy’ Answer the following questions: (Any two) 14
29. Q1 Explain different techniques used for screening of genomic library
30. Q2 Elaborate on construction of cDNA library
31. Q3 How can one achieve maximum expression of cloned gene? Elaborate on different strategies that can be applied to achieve the same Doas instructed: (Any six) 06
32. Q2 Fill in the blank: TALENs contain DNA cleavage domain named as What is the natural function of CRISPR? State the role of flurochrome in automated sequencing
33. Q5 Give significance of Piperidine
34. Q6 “Shotgun the blank: Si RNA is . (Single
35. Q8 State true or false: Fok 1 is a part of
36. Q9 State true or false: Maxam Gilbert method is based on enzymatic degradation by ddNTP
37. Q4 b. Answer the following questions: (Any Two) 14 marks
38. Q1 Describe the components and activity of CRISPR/Cas system
39. Q2 Explain the principle and method of Sanger's sequencing
40. Q3 Compare and contrast between ZFN and TALENs
41. Q5 Write short notes on the following: (Any four) 20 marks
    • a. DNA markers used for mapping human genetic variability
    • b. Pyrosequencing method
    • c. Cre-loxP recombination system for activating a gene
    • e. Improvement of seed quality protein
    • f. Chromosome walking

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