BSc Biotechnology Sem V 2018 2019 Nov 2019 BIOTECH GENOMES AND MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Give significance/role of: (Any six) 6 marks
4. Q2 Reporter gene
5. Q3 border sequences
6. Q4 G Protein
7. Q5 Micro Ti
8. Q7 Transgenic plants
9. Q8 site
10. Q9 Gemini virus
11. Q1 b. Answer the following questions: (Any two) 14 marks
12. Q1 Describe the mechanism of transfer of T-DNA in plants
13. Q2 “Particle gun method of gene delivery is an efficient method” - Justify
14. Q3 Give an account of seed quality protein improvement Explain the following: (Any six) 06
15. Q4 Xeno mouse
16. Q6 Male
17. Q9 Pseudopregnant female
18. Q2 b. Discuss the following questions: (Any two) 14 marks
19. Q1 Use of Cre- loxP recombination to regulate the expression of transgene
20. Q2 Embryonic stem cell method of producing transgenic mice
21. Q3 Cloning livestock by nuclear transfer Doas Directed: (Any six) 06
22. Q1 Define: shuttle vectors
23. Q2 State the importance of phagemid vectors
24. Q3 Name the enzyme used to form a phosphodiester bond between DNA
25. Q4 importance of ‘chromosome walking’ chromosomal library
26. Q5 State the significance of ‘western blotting’
27. Q9 one method of gene transfer in E. coli Answer the following questions: (Any two) 14
28. Q1 Elaborate on “Southern blotting’ for genomic library screening
29. Q2 What are ‘PET vectors’? Explain in detail
30. Q3 Explain the use of pUC vectors in recombinant DNA technology
31. Q4 a. Attempt the following objective questions as directed: (Any six) 6 marks
32. Q2 What is PAM ? Explain the term “gene
33. Q4 State true or false: Hydrazine is used in automated DNA sequencing State true or false: CRISPR/Cas9 system induces single stranded break
34. Q6 What are TALENs ?
35. Q7 Fill in the blank: Automated DNA sequencing different labels for detection of order of nucleotides
36. Q8 the blank: Chain termination method is based on incorporation of
37. Q9 What is the role of tracrRNA?
38. Q4 b. Answer the following questions (Any two) 14 marks
39. Q1 Compare and contrast between Maxam Gilbert’s method and Sanger's
40. Q2 Discuss the mechanism of RNA interference and state its significance
41. Q3 Illustrate the activity and advantages of Zinc finger nucleases
42. Q5 Short notes on the following: (Any four) 20 marks
    • a. Recent techniques for human genetic mapping
    • b. Applications of CRISPR/Cas system
    • c. Transgenic Fish
    • d. Vectors for plant cells
    • f. Cosmid vectors

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