BSc Biotechnology Sem III ATKT 2015-16 ATKT Biotech Question Paper - Mumbai University | munotes

Questions asked in this paper

• 3) Figures to the right indicate full marks

1. Q1 Synthesis of new DNA take place in the direction. to 3', 3' to 5', to
2. Q2 In Messelson and Stahl's experiment is the heavy isotope used as nitrogen source in growth medium for E.coli
3. Q3 The new DNA strand being made in the 5' to 3' direction same as movement of the replication fork is called the strand. (leading, lagging, template)
4. Q4 DNA replication takes place in the form of type of replication
5. Q5 enzyme is a form of topoisomerase which relaxes the tension produced in the DNA a head of the replication fork. (Gyrase, Helicase, Amylase)
6. Q6 DNA polymerase I removes RNA primer‘with its activity
7. Q7 DNA the initiation of DNA chain is carried out by using
8. Q8 enzyme is used to untwists the DNA to produce single stranded template
   • b) Explain (any two) of the following terms. 4
9. Q1 Okazaki fragments
   • c) Answer (any two) of the following. 12
10. Q1 Explain Looped Rolling Circle Model of DNA replication
11. Q2 Explain Bidirectional DNA replication
12. Q4 Explain the process of Initiation of DNA replication in E.coli
    • a) State whether the following statements are True or False (Any two). 2
13. Q1 In depurination a purine, either adenine or guanine is removed from the DNA DNA polymerase removes wrong nucleotide by its exonuclease proofreading activities
14. Q3 A common base analogy mutagen is 5-bromouracil
15. Q4 Photo reactivation occurs when and enzyme called amylase is activated by a photon
    • b) Explain (any three) of the follawing ce) Explain (any two) of the following
16. Q2 Repair by Methyl - Directed mismatch repair
17. Q4 Mismatch repair by DNA polymerase proof reading activity
18. Q3 a) Define the terms (Any two)
    • b) Give symbols used in pedigree for (any four) of the following
19. Q3 Male 4) Death
    • c) Attempt (any two) of the following. 12
20. Q1 Describe the formation of PD, NPD and TT tetrads at four strand chromatid: stage for linked genes
21. Q2 How will you establish gene order using a three point cross, explain with a suitable
22. Q3 Solve: A Neurospora strain that required argenine (arg) but can synthesize methionine (met+) for growth was mated to a strain (arg+ and met) following products was obtained. Compute the gene and centromere distance for the two genes
23. Q4 Observe the following pedigree and answer the following questions,
    • i) Which individual is the proband?
    • ii) What is the mode of inheritance?
    • iii) What is holandric trait?
    • iv) What are the characteristics of the condition hypertricosis?
    • v) The hormone found in males for expressing secondary sexual characteristics?
    • vi) What is hemizygosity?
24. Q4 Write short notes on (any three) of the following. 15 marks
25. Q1 Life cycle of Neurospora crassa
26. Q2 Importance of pedigree analysis
27. Q4 Role of DNA polymerase III during DNA replication
28. Q5 Sigma mode of DNA replication
29. Q6 Physical and chemical mutagens

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