BSc Biotechnology Sem III 2018 2019 2019 Biotech Biophysics Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 For Q 3 and Q 4 attempt A and B OR C and D
4. Q1 Do as directed (Any fifteen) 15 marks
5. Q1 Define the term ‘dispersion of light’
6. Q2 Who first demonstrated the phenomenon of interference of light?
7. Q3 What does the term ‘active medium’ in a laser refer to?
8. Q4 In colorimetric assay, a plot of concentration of standards versus their respective absorbance is called
9. Q5 The focusing of electron beams in an electron microscope is done using
10. Q6 microscopy allows the localisation of molecules within the cellular microenvironment for TEM and on the cell surface for
11. Q7 Give any one application of UV-Vis spectroscopy
12. Q8 The maximum displacement of a vibrating particle from its position of rest is called its
13. Q9 Ina wave the particles vibrate at right angles to the direction of propagation of the wave
14. Q10 In type of magnetism, the strength of the resultant magnetic field decreases on application of an external magnetic field the term ‘viscosity of a fluid.’
15. Q12 The ability of a liquid to maintain contact with a solid surface is called
16. Q13 The unit used for measurement of magnetic
17. Q14 The principle mode of heat transfer in solids is
18. Q15 Acronym APS in PAGE/SDS-PAGE stands for
19. Q16 The support matrix in PAGE is
20. Q17 Agarose is obtained from
21. Q18 Define:
    • Q. P. Code: 23075 Define : ‘tracking dye’ as used in electrophoresis
22. Q20 Name any one stain used to detect proteins on PAGE
23. Q2 A_ Explain construction of single and double beam spectrophotometer. Compare the properties of a laser light against normal light. 07 Explain sample preparation for SEM and TEM. 08 8 marks
24. Q2 D_ Explain the laws governing light absorption. Write a note on its significance in quantitative estimation of molecules 7 marks
25. Q3 A_ What is a thermocouple? Explain its use in measurement of temperature. Enlist the applications of ultrasound waves. 07 Describe the construction and use of an Ostwald’s viscometer. 08 8 marks
26. Q3 D_ Give an account of different types of magnetism observed in matter. Differentiate between AGE and PAGE. 08 7 marks
27. Q4 B Describe the general technique of paper electrophoresis. 7 marks
28. Q4 C_ Explain the general principle of electrophoresis What is native-PAGE? Explain its principle. 07 8 marks
29. Q5 — Write Short notes on any three of the following b Characteristics of electromagnetic waves 15 marks

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