BSc Biotechnology Sem III ATKT 2015-16 ATKT Biotechnology 3 Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 (A) Fill in the blanks -(Any four)
   • 1. The DNA synthesis when incorrect nucleotide is inserted. (3’-5’, 5’-3’, 4’ NaOH). periment, a solution of form a density gradient. (CsCl, Pr oteins bind to the single strand DNA for a preventing it lengthened by to synthesis new DNA strand during new strand that is made in the direction opposite that of the movement of the replication fork is called the strand. (Lagging, leading, template) enzyme is a form of topoisomerase which relaxes the tension produced in the DNA a head of the replication fork. (Gyrase, Helicase, Amylase)
2. Q1 (B) Explain the following terms. (any two) Answer any two of the following. (12)
   • 2. Explain the process of Initiation of DNA replication in E. coli
   • 3. Explain Rolling circle mode of DNA replication
   • 4. Explain the process of primer removing and fragment joining during DNA replication
   • (I) State whether the following statements are true or false. (any two). 2
   • 1. Deamination is the process of removal of an amino group from a base
   • E. coli, alkylation damage can be repaired by an enzyme called methylguanine methyltransferase which removes methyl group 4, In depurination, a pyrimidine either adenine or guanine is removed from the DNA
   • S.Y.B.Sc. SEM Explain the following terms. (any three) Answer any two of the following. (13)
   • 1. Write in brief about the mutation caused by Base modifying agents such as an
   • 2. Write in brief about repair mechanism by Photo reactivation and Alkylation damage,
   • 3. Repair by Methyi-Directed Mismatch repair
   • 4. Write a note on SOS Response Q (A) Explain the terms (any three) (6)
3. Q11 I B) Give symbols used in pedigree for (any two) of the following Q DI C) Attempt (any two) of the following (12) 1, Describe the formation of PD, NPD and TT tetrads at four strand chromatid stage tor 2 marks
   • 2. How will you establish gene order using a three point cross, explain with a suitable
   • 3. Solve:-A Neurospora strain that required argenine (arg) but can synthesize methionine (met +) for growth was mated to a strain (argt and met) following products were obtained. Compute the gene and centromere distance for the two genes Observe the following pedigree and answer the following questions: Which individual is the proband?
   • ii. What is the mode of inheritance? iil. What is holandric trait? IV. What are the characteristics of the condition hypertricosis? V. The hormone found in males for expressing secondary sexual characteristics?
   • vi. What is hemizygosity? V. Write a note on (any Three) of the following. (15)
   • 1. Mismatch repair by DNA polymerase proofreading
   • 5. Holliday model of recombination
   • 6. Life cycle of Neurospora

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