BSc Biotechnology Sem III 2023 2024 2024 TECHNOLOGY MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 For Q2,Q3 and Q 4 attempt A and B OR C and D directed (Any fifteen) 15
3. Q2 DNA strand that is ready to make an RNA strand is called the___ strand
4. Q3 sequence is always present coding sequence. (upstrem,
5. Q4 The consensus sequence is generally found at the -10 region, formerly called the
6. Q5 The product of transcription in the eukaryotic called mRNA (precursor, short, long, straight)
7. Q6 Promoter proximal elements in eukaryotes at -90 consensus GGGCGG sequence is
8. Q8 Name two functional groups present in amino acids
9. Q9 What are the two post-transcriptional modifications that occur in tRNA?
10. Q10 What is the size of larger and smaller ribosomal subunits in eukaryotes?
11. Q11 | Name three sites of translation present in ribosomes Which is the first step of elongation of translation? examples of initiation factors of eukaryotes
12. Q14 true or false. Once the signal sequence is fully into the cisternal space of the ER, it is removed from the polypeptide by the enzyme signal peptidase True or false: Permease enzyme ( of lac operon ) is also called M protein
13. Q16 | What do you mean by effector molecule?
14. Q17 What is the full form of CAP?
15. Q18 Define genetic switch Enlist the proteins which are required for the establishment of lysogenic pathway
16. Q20 Give an example of an inducible operon Describe in detail about elongation in the RNA chain in bacteria
17. Q2 B_ Explain the process of termination of transcription
18. Q2 C_ Explain in detail assembly of the transcription initiation machinery in eukaryotes. VCD/ SYBT SUB-MOLECULAR BIOLOGY IIT 2%Hours 75 Marks 8 marks
19. Q2 D Write a note on processing of pre-mRNA to Mature mRNA. 7 marks
20. Q3 A_ Explain in detail the elongation step of translation in bacteria. 8 marks
21. Q3 B_ Discuss the initiation of translation in eukaryotes. 7 marks
22. Q3 C_ Write a note on four levels of structural organization of protein. 8 marks
23. Q3 D_ Explain in detail the structure and composition of the bacterial ribosome. What is the attenuation of trp operon? Explain in detail about the attenuation model 08 7 marks
24. Q4 B_ Write a note on ara operon with positive and negative control of its regulation 7 marks
25. Q4 C What is a genetic switch? Explain in detail about lytic pathway regulation of phage. How is the lysogenic pathway established as bacteriophage? Explain in detail 07 Write short note on any three of the following 15 a 5’ capping in eukaryotes b 3’ modification in eukaryotes d Role of lac Operon enzyme 8 marks

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