B.Sc. (Biotechnology) Sem III ATKT BIOTECH MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 and Q4 attempt A and B OR C and D
4. Q1 Do as directed (Any fifteen) 15 marks
5. Q1 Define enhancer elements
6. Q2 State the significance of TATA box
7. Q3 State true or false: Sigma factor is required throughout the transcription
8. Q4 What are splisosomes ?
9. Q5 State function of RNA polymerase II
10. Q6 What does CPSF stand for?
11. Q7 What are introns?
12. Q8 is an enzyme which adds formyl group to methionine during initiation of translation in bacteria
13. Q9 Give the significance of A-site in translation process
14. Q10 Ubiquitin tags protein to target it for degradation. (State true or false) What are sense codons?
15. Q12 The m-RNA ribosome binding site region in bacteria is commonly known as the sequence
16. Q13 Each m-RNA codon that specifies an amino acid in a polypeptide chain consists of nucleotides
17. Q14 During protein sorting, once the signal sequence is fully into the cisternal space of the endoplasmic reticulum, it is removed from the polypeptide by the enzyme
18. Q15 All of the genes involved in functioning of the lac operon are inducible (State True or False)
19. Q16 Define : Catabolite repression cro protein and repressor proteins bind to the same sites within OR (True or False)
20. Q18 Anthranilate synthetase component I is coded by gene
21. Q19 is a phenomenon whereby a gene is transcriptionally silent due to its location and not because of the action of a specific repressor
22. Q20 Define miRNA
23. Q2 A_ Explain initiation of transcription in prokaryotes What is 5' capping? Explain. 07 Explain termination of transcription in eukaryotes. 08 8 marks
24. Q2 D_ Explain Rho independent termination of transcription. 7 marks
25. Q3 A_ State the steps involved in elongation phase of translation. Explain any two steps in detail Draw the structure of t-RNA. Explain the role of t-RNA in translation. 07 the translocation of proteins into endoplasmic reticulum in 08 eukaryotes with the help of a suitable diagram any four characteristics of genetic code. Explain wobble’s hypothesis 07 with an example Elaborate on the molecular model for attenuation of the trp operon. 08 Write a note on mutants of lac operon. 07 Give an account of positive regulation of lac operon. 08 Discuss regulation of transcription initiation by activators and repressors. 07 8 marks
26. Q5 Write Short notes on any three of the following 15 marks
    • a. Gene silencing
    • b. Genetic switch in lambda phage
    • c. Degeneracy of Genetic code
    • d. Amino-acylation of t-RNA
    • e. RNA editing

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