BSc Biotechnology Sem III 2018 2019 2019 Biotech Bioprocess Technology Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 and Q4 attempt A and B OR C and D Doas directed (Any fifteen) 15
4. Q1 Give one example of fungi used as protein supplement
5. Q2 State any one disadvantage of serial subculture method used for preservation of
6. Q3 What is the role of chemical, ‘phosphorus pentoxide’ in process of lyophilisation?
7. Q4 Give role of “giant — colony” technique
8. Q5 How volatile organic compound as a carbon source is provided to microorganisms degrading it during screening?
9. Q6 State any one objective of secondary screening
10. Q7 Name the method suitable for preservation of microorganism in the laboratory with relatively limited funds and small collection
11. Q8 Give one example of chelating agent used in fermentation media
12. Q9 State the role of baffles in the fermenter the organism used in industrial ethanol production
13. Q11 Phenyl acetic acid is used as a in penicillin production State the use of paramagnetic gas analyser
14. Q13 method is used universally for sterilization of fermentation Name any one type of continuous sterilizer used for sterilization of fermentation two techniques that provide valuable information on the chemical structure of the fermentation product
15. Q16 the blank: Fluorescent dye allows microscopic counting of only viable cells by fluorescent microscope
16. Q17 What do you mean by Pharmacokinetics?
17. Q18 the protozoan employed in bioassay of Vitamin B12
18. Q19 Enlist two metabolic reactions used for metabolic response assays
19. Q20 true or False: Difference between minimum effective dose & minimum toxic dose is referred as Therapeutic index
20. Q2 A. Discuss primary screening of antibiotic producing microorganisms. 8 marks
21. Q2 B. brief account on any two industrial processes which involve application of — 7 marks
22. Q2 C. Describe process of lyophilisation to preserve industrially important strains. How the method is different from method of cryopreservation?
23. Q2 D. What is secondary screening? Differentiate it from primary screening. 7 marks
24. Q3 A. Discuss the role of different components in fermentation media. 8 marks
25. Q3 B. Describe batch sterilization of fermentation media. State the advantages and disadvantages of the method 7 marks
26. Q3 C. Schematically explain industrial production and recovery of penicillin. 8 marks
27. Q3 D. brief account on submerged fermentation using suitable example. State the advantages and disadvantages of the process 7 marks
28. Q4 A. Describe Thin Layer Chromatography as a method of analysis of product from Discuss turbidimetric & growth assays for analysis of fermentation broth. 07 8 marks
29. Q4 C. Elaborate on Enzymatic methods for analysis of fermentation broth. Give an account on Blood concentration-time profile for a theoretical drug given 07 Short notes on any three of the following 15 8 marks
    • b. Industrial applications of algae Cc. Filter sterilization of fermentation media
    • d. Methods to measure dissolved oxygen in a fermentor
    • e. Bioequivalence & it’s assessment

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