BSc Biotechnology Sem III 2023 2024 2024 BIO TECHNOLOGY APPLIED CHEMISTRY I Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q2 All questions carry equal marks For Q 2, Q3 and Q 4 attempt A and B OR C and D directed (Any fifteen) 15 Molecular weight of hemoglobin is enzyme proteins containing metai ions which are directly bound to the protein or to non protein components
2. Q3 Name the enzyme catalyzes the decomposition of hydrogen peroxide to water and oxygen
3. Q4 What mineral is needed for wound healing? When a and b have added to the C=C double bond of the substrate from the same side is a addition
4. Q6 Define Enzymes
5. Q7 macroelements are required in amounts than 100 mg/day
6. Q8 Give one example of Cation Exchanger
7. Q9 fixed-angle rotors, the sample tubes are held fixed at the angle of the separation of nucleic acids in a CsCl gradient is an example of the function of the Elution buffer Give an example of a density gradient solution used for separation of
8. Q13 Give an ONE property of a ligand used in Affinity chromatography
9. Q15 Which 2 key steps are involved in a 2D gel electrophoresis
10. Q16 Define Gel Documentation Which stain is commonly used to visualize proteins on an SDS-PAGE gel?
11. Q18 is the migration of charged particles or molecules in a medium under the influence of an applied electric field
12. Q19 is the purpose of B-mercaptoethanol in SDS-PAGE?
13. Q20 Filter paper as a stabilizing medium is very popular for the study of normal and abnormal plasma proteins. (True False) vcD/ Ill SUB: PAPER CHEMISTRY | 75 MARKS 2HRS 30 MIN
14. Q2 A Explain the Difference between and Myoglobin. What is an addition reaction? Explain its types. 07 8 marks
15. Q2 C What are Microminerals? Explain iti detail any 3 Microminerals. Q What is the role of catalase enzymc? Explain its Application 8 marks
16. Q3 A Give an account on principle and applications of Ion exchange Elaborate on working and applications of differential centrifugation. 07 8 marks
17. Q3 C Explain the priaciple and applications of Size exclusion chromatography. Derive the equation of Relative Centrifugal Force. 07 8 marks
18. Q4 A Explain Agarose Gel Electrophoresis and it's applications . Explain the difference between AGE and SDS PAGE. 07 8 marks
19. Q4 C Explain Paper electrophoresis with a suitable diagram . What are the factors which can affect electrophoretic mobility ? 07 8 marks
20. Q5 Short notes on any three of the following 15 marks
    • b. Heme Group
    • d. Applications of Electrophoresis Migration of ions in an applied electric field

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