BSc Biotechnology Sem V ATKT BIOTECH MEDICAL MICROBIOLOGY & INSTRUMENTATION Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Doas instructed: (Any six) 6 marks
4. Q1 Define pock
5. Q2 What is a Lethal Dose?
6. Q3 Give one example of plant disease caused by viroids
7. Q4 State the role of spikes
8. Q5 State true or false: Virus replication inside a cell usually causes death or disease of that cell
9. Q6 Name any one site in an embryonated egg that is used to inoculate
10. Q7 the blank: Viruses that cannot multiply unless they invade a specific host cell are called as
11. Q8 What is nucleocapsid?
12. Q9 Give one example of an enzyme used to degrade cellular ribonucleic acids and proteins Give an account of the following: (Any Two) 14
13. Q1 General structures of Virus
14. Q2 Cytocidal infection and the mechanisms of host cell damage
15. Q3 Reproduction of dsDNA phages
16. Q2 a. Give one example of each: (Any six) 6 marks
17. Q1 Antifungal drug
18. Q4 Competitive inhibitor
19. Q8 Sulpha Drug
20. Q2 b. Elaborate on the following questions: (Any Two) 14 marks
21. Q1 Mode of action of Penicillin and Cephalosporins on bacterial cell wall
22. Q2 Action of Nucleic acid synthesis inhibitors
23. Q3 Origin and transmission of drug resistance
24. Q3 a. Attempt the following objectives as directed: (Any six) 6 marks
25. Q1 Define Stokes shift
26. Q2 Name the instrument which is used in the study of photosynthesis and respiration in plants
27. Q3 Sate the role of microspectrofluorimetry
28. Q4 What is intrinsic fluorescence?
29. Q5 Fill in the blank: For analysis of drug metabolites, IR is coupled with
30. Q6 What is pre-filter absorption?
31. Q7 true or false: The presence of tryptophan and FAD as cofactors allows fluorescence to be measured in proteins
32. Q8 Name the protein used for determining calcium ion concentration by
33. Q9 Give the full form of FT-IR Answer the following questions: (Any Two) 14
34. Q1 principle any two applications of Fluorescence
35. Q2 Elaborate on Quasi-elastic light scattering and its applications Give an account on Instrumentation and applications of Atomic
36. Q4 a. Doas directed: (Any Six) 6 marks
37. Q1 What is affinity chromatography?
38. Q2 State true or false: Calmodulin is a regulatory binding proteins is present in all eukaryotic cells is used as ligand for eluting proteins
39. Q3 the blank: In method validation, precision is expressed in terms of . (Mean, Relative standard deviation, regression coefficient)
40. Q4 What is a Dead time of a counter?
41. Q5 Name any one column used in HPLC
42. Q6 What is method validation?
43. Q7 Define linearity
44. Q8 Give any one disadvantage of scintillation counting
45. Q9 What is an autoradiography? Discuss the following : (Any Two) 14
46. Q1 With reference to Size exclusion chromatography:
    • a. Principle and working
    • b. Any three applications
47. Q2 With reference to isotopes in biology:
    • a. Nature of radioactivity decay
48. Q3 With reference to Ion exchange chromatography:
    • a. Principle
    • b. Anion and Cation exchangers
    • c. Any two applications Write Short notes on the following (Any four) 20
    • a. Viral Capsid
    • b. Cultivation of bacterial viruses Principle of IR
    • e. Use and misuse of antimicrobial drugs
    • f. Action of antiviral drugs

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