BSc Biotechnology Sem V ATKT BIOTECH BIOTECHNOLOGY PAPER IV INDUSTRIAL BIO TECHNOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory
2. Q2 All questions carry equal marks
3. Q1 a. Doas directed. (Any three) 3 marks
4. Q1 Name a fermenter used for production of vinegar
   • ii) Define on-line sensor lil) Name any one gauge used to measure pressure fermenter
   • iv) What are perfusion cultures?
   • v) Fill in the blank is a device for introducing air into the liquid in a fermenter Vi) State any one agent used for foam control
5. Q1 b. Answer the following. (Any two) 12 marks
6. Q1 Describe construction, principle and working of a deep jet fermenter li) Briefly explain the measuring and control of temperature in a lil) Elaborate on hollow fibre chambers and perfusion cultures with respect to animal cell cultures
   • iv) How can you measure and control inlet and exit gas?
7. Q2 a. Explain the following terms. (Any three) 3 marks
   • v) Crop Answer the following. (Any Two) 12
8. Q1 Elaborate on the aspects to be considered in the scale down of pilot plant li) Describe the development of mycelial inoculum by sporulation on a solid medium with a suitable example lil) Discuss development of inocula for yeast process with suitable examples Explain citing suitable example the stages involved in secondary screening of an antibiotic producer Give significance of /importance of (Any three) 03
9. Q1 Mashing Hops in Beer
   • v) Clarification Vi) Boiling of wort
10. Q3 b. Elaborate on (Any two) of the following. 12 marks
    • i) Production of White wine li) Submerged culture method for Citric acid production ili) Fermentation of Beer
11. Q4 a. Mention the application/role of the following in downstream processing (Any three) 3 marks
12. Q1 Tangential filtration
13. Q4 b. Give an account of the following with respect to product recovery 12 marks
    • i) Ultrafiltration and reverse osmosis li) Two types of chromatography techniques used lil) Any two types of continuous filters
    • iv) Drying - any two methods short notes on the following. (any three) 15
    • i) Measurement of biomass ili) Criteria involved in strain selection for industrial fermentation Vi) Any two chemical methods for cell disruption

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