BSc Biotechnology Sem V 2018 2019 Nov 2019 BIOTECH MEDICAL MICROBIOLOGY AND INSTRUMENTATION Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Doas instructed: (Any six) 6 marks
4. Q1 Inclusion bodies
5. Q2 is Infectious Dose?
6. Q3 one example of plus strand RNA virus
7. Q4 State true or false: Viruses are inactive macromolecules outside of the host cell and active only inside host cell
8. Q5 in the blank: The HIV virus, which cause AIDS destroy
9. Q6 Give one example of complex viruses
10. Q7 Name the virus that attacks tobacco leaves
11. Q8 What are plaques?
12. Q9 the role of viral capsid
13. Q1 b. Give an account of the following: (Any Two) 14 marks
14. Q1 Cultivation of animal viruses and plant viruses
15. Q2 Baltimore Classification of virus
16. Q3 Hemagglutination assay and plaque assay Give one example of each: (Any six) 06
17. Q1 Antifungal drug
18. Q2 Drug causing injury to plasma membrane
19. Q5 Bacteriostatic drug
20. Q9 Antimetabolic drug
21. Q2 b. Elaborate on the following questions: (Any Two) 14 marks
22. Q1 Mode of action of Protein sysnthesis inhibitors with example
23. Q2 Origin and transmission of drug resistance in bacteria
24. Q3 Beta-lactum antibiotics and its action of cell wall synthesis inhibition
25. Q3 a. Attempt the following objectives as directed: (Any six) 6 marks
26. Q2 one example of radiation source used in spectrofluorimeter
27. Q3 What is a Golay cell?
28. Q4 in the blank: Atomic absorption spectrophotometer is used in clinical laboratories, for the determination of in body fluids
29. Q5 Name the instrument in which no monochromator is used
30. Q6 the role of two monochromators used in spectrofluorimeter
31. Q7 What is fluorescence bleaching?
32. Q8 Give the working wavelength range for atomic absorption spectroscopy
33. Q9 Give one advantage of spectrofluorimetry over spectrophotometer Answer the following questions: (Any Two) 14
34. Q1 Discuss on the principle and any two applications of luminometry
35. Q2 What is elastic light scattering? Elaborate on determination of molecular mass with multi-angle light scattering
36. Q3 Give an account on the Principle and applications of Atomic absorption
37. Q4 a. Doas directed: (Any Six) 6 marks
38. Q1 What is a cation exchange chromatography?
39. Q2 State true or false: Quaternary ammonium (Q) is a strong functional group used in anion exchangers
40. Q3 Fill in the blank: In size exclusion chromatography molecules will move at a faster rate in the column
41. Q4 is the use of salt gradient in ion exchange chromatography? Give an example of an alpha decay
42. Q6 any one emission of the most common forms of radioactive
43. Q7 Define Half life of an isotope
44. Q8 What is void volume in size exclusion chromatography?
45. Q9 What is an autoradiography?
46. Q4 b. Discuss the following : (Any Two) 14 marks
47. Q1 With reference to affinity chromatography:
    • a. Ligand, examples of ligand and spacer arm
    • b. Any three applications
48. Q2 With reference to Scintillation counting:
    • a. Principle and types
    • b. Two advantages and two disadvantages
49. Q3 With reference to High performance liquid chromatography:
    • a. Concept and steps of Method development
    • b. Validation parameters laid by ICH guidelines
50. Q5 Write Short notes on the following (Any four) 20 marks
    • b. Reproduction of TMV
    • d. GM counters
    • e. Selective toxicity of antibacterial agents

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