BSc Biotechnology Sem VI 2016 17 2016-17 Biotechnology II Biochemistry, Immunology, Instrumenation Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 Explain the term: (any one) 2 marks
   • i) Agglutinins
   • b) Give one example of: (any one) (01)
   • i) | Fluorescent compounds used in immunoassay
   • c) Answer the following (any two) 12
   • i) Outline the general features of antigen-antibody reaction
   • ii) Describe complement fixation test
   • iii) Explain the steps involved in sandwich ELISA
   • iv) Explain the working of Fluorescence-activated cell sorter and its application Answer in one word: (any three) (03)
   • i) Active form of androgen
   • ii) on which TSH act
   • iv) Cells of the testes that produce androgen
   • v) Transport protein of T3 and T4
   • vi) Hormone associated with Cushing’s syndrome
   • b) Discuss the following: (any two) 12
   • i) Biochemical functions of calcitriol
   • ii) Mechanism of action of group | hormones
   • iii) biochemical function of estrogen
   • iv). Release, transport and any two biochemical function of thyroid hormone the pathway to which the following molecules belong (any three) (03)
   • i) protein
   • v) Acetone
   • vi) ~Squalene
   • Q.P. Code: 01239
   • b) Attempt the following (any two) 12
   • i) the role of acetyl CoA carboxylase in lipid metabolism
   • ii) Schematically represent synthesis of TAG from glycerol
   • iii) Write the flow-sheet for formation of activated isoprene on cholesterol biosynthesis
   • iv) Describe the formation of ketone bodies in the liver
2. Q4 a) Explain the term: (any one) 2 marks
   • i) Curie
   • b) Give one example of: (any one) (01)
   • i) | Detector used in IR spectroscopy li) of radiation in fluorescent spectroscopy
   • c) Describe and give two applications of the following techniques (any two) 12
   • ii) Working of confocal microscope
   • iii) used in fluorescent spectroscopy
3. Q5 Write short note on (any three) 15 marks
   • a. Coomb’s test
   • b. and application
   • c. Menstrual cycle
   • d. Abnormalities of thyroid function
   • e. Types of radioactive decay
   • f. Transcriptional regulation of cholesterol biosynthesis

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