BSc Biotechnology Sem VI 2016 17 2016-17 Biotech Molecular Biology & Bioinformatics Question Paper - Mumbai University | munotes

Questions asked in this paper

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• N.B: 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q1 a. Give the role/application of (any three) 3 marks
   • i. Probe
   • ii. VNTR
   • iii. RFLP
   • iv. Primers
   • v. Peptide vaccine
   • b. Discuss the following (any two) 12
   • i. Southern Hybridization
3. Q2 a. Explain the significance of the following (any three) 3 marks
   • i. Opines
   • ii. T-DNA
   • iii. Disarming
   • v. PEG
   • b. Attempt the following (any three) 12
   • i. Give an account of Bt cotton and its applications
   • ii. Give an account of ‘Microprojectile a method of producing transgenic plants’
   • iii. Schematically explain the production of T-DNA copy
   • iv. What are edible vaccines? State their advantages
4. Q3 a. Define the following (any three) 3 marks
   • iii. Transgene
   • v. cre gene
   • b. Give an account of the following (any two) Use of Cre- loxP recombination to regulate the expression of transgene 12
   • ii. Embryonic stem cell method of producing transgenic mice Transgenic mouse as model system to study human disease
   • iv. Cloning livestock by nuclear transfer
   • Q.P. Code :01208
5. Q4 a. Explain of the following terms (any three) 3 marks
   • i. Book mark
   • iii. DDBJ
   • iv. WWW
   • v. IP address
   • vi. BLAST
   • b. Answer the following (any two) 12
   • i. Discuss: NCBI and its importance in Bioinformatics
   • ii. What are Data retrieval tools? Give an account of the data retrievals tools and
   • iii. Write a note on Internet and its protocols associated with Bioinformatics
   • iv. Elaborate on: Protein databases
6. Q5 Write short notes on (any three) 15 marks
   • b) Cholera vaccine
   • d) Mode of action of Bt toxin
   • e) Transgenic fish as biosensors of environmental pollutants
   • f) Applications of Bioinformatics

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