BSc Biotechnology Sem VI 2016 17 2016-17 Biotech Pri. Of Gene Cloning Question Paper - Mumbai University | munotes

Questions asked in this paper

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• N.B: 1. Attempt all questions

1. Q2 All questions carry equal marks
   • a. Give an example of the following: (any three) 3
   • i) Cofactor required for T4 ligase
   • ii) | Source of Alkaline phosphatase
   • iii) DNase
   • iv) used for probe labelling
   • v) Enzymatic reaction exhibited by Polynucleotide kinase
   • b. Answer of the following: (any two) 12
   • i) | Give an account of nature of cut ends generated by restriction endonucleases
   • ii) | What are linkers and adapters? State their role in rDNA technology
   • iii) Comment: Terminal Transferase has many applications
   • iv) Elaborate on the different applications of T4 DNA polymerase
2. Q2 a. Explain the following terms: (any three) 3 marks
   • iv). T-DNA
   • b. Attempt the following: (any two) 12
   • i) pBR322 is an ideal cloning vector. Justify
   • ii) an account of: Lambda phage as a vector
   • iii) Explain the construction of M13 phage based vectors
   • iv) Describe binary cloning vector with the help of a neat labeled diagram
3. Q3 a. Give the importance of the following: (any three) 3 marks
   • vi) ddNTP
   • Q.P. Code :01672
   • b. Give an account of the following: (any two) 12
   • i) | Construction of genomic DNA library
   • ii) Identification of clones in cDNA library
   • iii) fingerprinting
   • iv) Any two types of PCR
4. Q4 a. State the significance of the following: (any three) ii) 3 marks
   • iii) Islet of Langerhans
   • b. Discuss the following: (any two) 12
   • i) Limitations of traditional methods of vaccine production
   • ii) | Attenuated vaccine for Cholera
   • iv) Diagnosis of Sickle cell anemia
5. Q5 Write short notes of the following: (any three) 15 marks
   • i) and applications of PCR
   • ii) Features of an ideal cloning vector of reverse transcriptase in rDNA technology
   • v) Klenow fragment

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