BSc Biotechnology Sem VI ATKT BIOTECH BIOTECHNOLOGY PAPER II BIOCHEMISTRY IMMUNOLOGY AND INSTRUMENTATION Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 (a) State two applications of the following: (any one) 2 marks
   • (b)Give one example of the following: (any one)
   • (i) Incomplete antibody
   • (c) Answer the following: (any two) 12
   • (i) Give an account of working of Fluorescence-activated cell sorter and give any two of its applications
   • (ii) Outline the general features of antigen-antibody reaction Explain indirect and sandwich ELISA
   • (iv) direct and indirect Coomb’s test
2. Q2 (a) Name the following: (any three) 3 marks
   • (i) Any one hormone produced by Zona glomerulosa
   • (ii) Disorder associated with hypothyroidism in children
   • (iii) Hormone associated with Addison’s disease
   • (iv) hormone in the luteal phase of menstrual cycle
   • (v) Transport protein for thyroid hormone
   • (vi) Anexample of a C-18 steroid hormone of the gonads
   • (b) Answer the following: (any two) 12
   • (1) Menstrual cycle is a good example of coordination among hormonal functions
   • (ii) Discuss the physiological and any three biochemical functions of DHT
   • (iii) Describe the effects of thyroid hormones on metabolism
   • (iv). an account of the biochemical functions of cortisol
3. Q3 (a) Identify the pathway to which the following enzymes belong: (any three) 3 marks
   • (i) Acetoacetate decarboxylase 20BE3C4B76762B397A54AB3C6B20CB40
   • Q.P. Code :32312
   • (b) Attempt the following questions: (any two) 12
   • (i) Schematically represent the reactions of fatty acid biosynthesis
   • (ii) Describe the reactions for biosynthesis of Triacylglycerol from DHAP
   • (iii) Write the flow-sheet for conversion of activated isoprene to cholesterol
   • (iv) Describe the cellular strategies for attaching phospholipid head group Do as directed: (any three) 03
   • (i) Define positron emission
   • (ii) Give an example of source of radiation in electron microscope
   • (iii) State True/false: Thermocouples are used as detectors in spectrofluorimeters
   • (iv) in the blank: is the commonly used unit for radioactivity
   • (v) Choose the correct option: stained specimens are observed in confocal
   • (vi) true/false: Infrared spectrophotometry can be used to study protein
   • (b) Attempt the following: (any two) 12
   • (i) Describe the process of image formation in confocal microscopy
   • (ii) Discuss the working of liquid scintillation counter
   • (iii) What are basic components of spectrofluorimeter? Briefly discuss its working
   • (iv) Radioactive isotopes have a wide range of applications in biological
4. Q5 Write short notes on: (any three) 15 marks
   • (a) Regulation of fatty acid synthesis
   • (b) Role of 8 Hydroxybutyrate as.a fuel
   • (c) Specimen preparation in TEM
   • (d) Disorders associated with deficiency of calcitriol
   • (e) Mechanism of action of group I hormones 20BE3C4B76762B397A54AB3C6B20CB40

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