BSc Biotechnology Sem VI 2016 17 2016-17 Biotech Paper III Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q2 All questions carry equal marks
   • a. State the application of the following: (any three) 3
   • i) Star activity iii)
   • iv) Adapters
   • v) fragment
   • b. Answer of the following: (any two) 12
   • i) | Give an account of mode of action of reverse transcriptase
   • ii) | Schematically explain random priming
   • iii) | Comment: Nucleases have many applications
   • iv) Give an account of sources and applications of DNA ligases
2. Q2 a. Explain of the following terms: (any three) 3 marks
   • i) Shuttle vector
   • ii) gene
   • v) . Crown gall
   • b. Attempt of the following: (any two) 12
   • i) pUC19 is an ideal cloning vector. Justify describe the cloning of DNA in bacteriophage lambda
   • iii) Describe: Cointegrate vector as a Ti plasmid derived cloning vector
   • iv) Discuss: Types and applications of M13 based vectors
3. Q3 a. Give the importance of the following: (any three) 3 marks
   • ii) transcriptase iii)
   • iv) Sequenase
   • Q.P. Code :01674
   • b. Give an account of the following: (any two) 12
   • i) | Construction of a cDNA library
   • ii) | Gene sequencing by Maxam and Gilbert's method
   • iii) Principle and applications of PCR
   • iv) Screening of genomic library
4. Q4 a. State the significance of the following: (any three) 3 marks
   • iii) HBcAg
   • iv) Clal
   • v) gD protein
   • vi) RFLP
   • b. Discuss the following: (any two) 12
   • i) of genetic immunization over conventional vaccines
   • ii) | Subunit vaccine for herpes simplex virus
5. Q5 Write short notes of the following: (any three) 15 marks
   • ii) and applications of DNA fingerprinting
   • iii) Types and application of lambda vectors
   • v) Diagnosis of Sickle cell anemia

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