BSc Biotechnology Sem VI 2018 19 May 2018-19 BIOTECH BIOTECHNOLOGY PAPER III MOLECULAR BIOLOGY & BIOINFORMATICS Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a Give an example of the following (any three) 3 marks
   • i. line used for HSV vaccine preparation
   • ii. Method used for DNA sequencing ili. Restriction enzyme used for detection of SCA
   • iv. Restriction enzyme used for preparation of attenuated Cholera vaccine
   • v. enzyme used in PCR
   • vi. Virus used for preparation of vector vaccine
4. Q1 b. Answer the following (any two) 12 marks
   • i. Discuss the role of VNTRs in solving paternity case li. Give an account of automated DNA Sequencing
   • iii. Give an account of peptide vaccines with suitable example
   • iv. What is subunit vaccine? State its advantages and disadvantages 2.a. Do as directed (any three): 3 li. State the action of Cry protein uli. Give significance of the gene coding for Phytoene synthase in production of golden
   • iv. Give significance of triparental mating while using Ti plasmid vector
   • v. What is mini- binary vector?
   • vi. Define: microprojectile 2.b. Attempt the following (any two): 12
   • i. Elaborate on the application of recombination DNA technology in improving the quality of food What is protoplast fusion? Explain its role in plant transgenesis ui. — Discuss: Ti plasmid mediated vector system
   • iv. Write a note on the ballistic method of DNA delivery to create transgenic plant the following terms (any three): 3 Elaborate the following (any two): 12
   • i. Cloning of sheep by nuclear transfer method li. Establishment of transgenic mouse with retrovirus vector
   • iii. Method of establishment of transgenic fish with enhanced growth rate 4.a. Explain the following: (any three) 3
   • i. Browser
   • iv. NCBI
   • vi. URL 4.b. Answer the following (any two): 12
   • i. Give a detailed account of scope and applications of Bioinformatics
   • ii. What is internet? Explain the role played by internet in Bioinformatics uli. Discuss: Tools used for sequence alignment
   • iv. Elaborate on DNA databases
5. Q5 Write short notes on (any three): 15 marks
   • iv. Applications of PCR
   • v. Applications of transgenic mice
   • vi. Establishment of transgenic mouse with microinjection

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