BSc Biotechnology Sem VI ATKT 2018-19 ATKT Biotech III Principles Gene Cloning & Recombined DNA Tech 19 Old Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All questions are compulsory
2. Q2 All questions carry equal marks
3. Q1 a. Explain the following terms (any three): 3 marks
   • b. Give an account of the following (any two): 12
4. Q1 Mode of action of RNA directed DNA polymerases li. Source and mechanism of action of Polynucleotide kinases Types of nucleases and its role in rDNA technology Iv. Alkaline phosphatase and its applications
5. Q2 a. Explain the following terms (any three): 3 marks
   • b. Discuss the following (any two): 12 Criteria to design an ideal cloning vector li. Shuttle vectors with a suitable example
   • iii. Construction and application of cosmid cloning vectors
   • iv. Advantages of pBR322 Doas directed (any three): 03 1 Define genomic libraries li State the significance of ‘hydrazine’ in Maxam- Gilbert method of DNA ili Define ‘primer’ iv Fill in the blank: Northern hybridization involves the transfer of from gel to nitrocellulose membrane
   • Q.P. Code: 31702 State true or false: Linkers are used to create sticky ends on blunt ended vi State the significance of Taq polymerase
   • b. Attempt the following (any two): 12
6. Q1 Elaborate on construction of cDNA libraries li. Explain Sanger’s method of DNA sequencing lil. Write a note on ‘Restriction mapping’ Iv. Describe screening of a genomic library
7. Q4 a. you understand by the following terms? (any three): 3 marks
   • b. Answer the following (any two): 12
8. Q1 Explain the use of restriction endonuclease in the detection of disease li. Differentiate between somatic and germ line gene therapy Give an account of the advantages of recombinant vaccines over iv Diagrammatically explain the preparation of Subunit vaccine against
9. Q5 short notes on of the following (any three): lil. Thymidine kinase marker in vaccine preparation Applications of DNA typing 15 marks

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