B.Sc. (Biotechnology) Sem V 2022 2023 Nov 2023 MEDICAL MICROBIOLOGY AND INSTRUMENTATION Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed Select the correct alternative (Any six) 06 Which of the following does the virus lack?
3. Q2 Baltimore’s classification of viruses has groups
4. Q3 — The replication of hepatitis B includes which of the following stages?
   • a. Movement of the intact virus to the cellular cytoplasm for replication
   • b. Conversion of relaxed circular viral DNA into covalently closed circular (CCC) DNA in the nucleus
   • c. Virions produced in the cytoplasm by cellular DNA polymerase
   • d. Oncogenic activity to transform liver cells Which of the following is the example of a negative single-stranded RNA animal
5. Q5 Plaque is formed
   • d. both bacteriophage and plant virus
6. Q6 strand of RNA virus
   • a. should be converted to m-RNA before it can be translated
   • b. is not recognized by host ribosomes
   • c. should convert to double-stranded DNA and then be expressed
   • d. can be directly translated to produce viral protein
7. Q7 An infection that results in cell death is referred to as
8. Q8 Nucleocapsid.of a virus is made up of
   • a. Nucleus and capsomers b. Envelope and protein
   • c. Protein and Nucleic acid d. viral nucleus
9. Q9 Icosahedron symmetry of the capsid has
   • a. 20 sides and 12 corners b. 12 sides and 6 corners
   • c.8 sides and 4 corners d. 10 sides and
10. Q1 b. Answer the following questions: (Any Two) 14 marks
11. Q1 Describe the three main techniques for cultivating viruses
12. Q2 Explain any two ways by which animal viruses are released by a host cell
13. Q3 How are prions different from viruses, and what are viroids?
14. Q2 a. Select the correct alternative (Any six) 6 marks
15. Q1 Which of these is a Beta-lactamase inhibitor? Which of the following classes of antibiotics bind to the 30S subunit of rRNA?
16. Q3 Which of the following is a drug that resembles deoxy-GTP and inhibits the virus are responsible for
    • a. cell membrane disruption b. inhibition of cell wall synthesis
    • c. inhibition of DNA synthesis d. inhibition of protein synthesis
17. Q5 is the agent when added to inhibits its growth and on removal the colony regrows
18. Q6 Nystatin is a drug used for the treatment of diseases caused by Which antimicrobial agent binds to DNA-dependent RNA polymerase and blocks the synthesis of mRNA?
19. Q8 Which of the following is used as a reverse transcriptase inhibitor in the treatment
20. Q9 of the following are true regarding metronidazole EXCEPT
    • a. It inhibits alcohol dehydrogenase b. It is used to treat giardia
    • c. It metallic taste in the mouth d. It is useful against trichomonoas
21. Q2 b. account on the following questions: (Any Two) The action of metabolic pathway inhibitors with a suitable example 14 marks
22. Q2 Mechanism of drug resistance in bacteria
23. Q3 Mode of action of Penicillin and Cephalosporins on bacteria
24. Q3 a. Select the correct alternative (Any six) 6 marks
25. Q1 to rotation and translation, macromolecules move into and out of a very small region in the solution this is called
26. Q2 of dynamic light scattering
    • a. Determination of diffusion coefficients c. Both b
    • b. Assessment of protein aggregation d. Neither a nor b atoms in atomic spectroscopy are vaporized by
    • d. All of these
27. Q4 In Atomic emission spectroscopy, small part.of the sample is vaporized so that
    • a. Every atomic component emits light at wavelengths corresponding to changing
    • b. Every atomic component emits light at no wavelength
    • c. Every atomic component does not emit light
    • d. Neither of these The primary spectrofluorimetry is placed in between
    • a. Source and cell b. Cell and detector
    • c. Source and detector Anywhere
28. Q6 detection of non-fluorescent compounds can be achieved by coupling a fluorescent probe, this is called
29. Q7 commonly used enzyme in bioluminescence is
30. Q8 The major disadvantage of spectrofluorimetry is
    • a. Time required b. Quenching Use of two monochromators
    • d. Inaccuracy
31. Q9 When the incident light beam hits a its ground state, there is a low probability that the molecule is excited and occupies the
    • a. Next higher vibrational state b. Next lower vibrational state
    • c. Heat energy d. Neither of these Discuss the following: (Any Two) 14
    • b. How molecular mass can be determined by multi-angle light scattering?
32. Q2 Explain the principle and applications of fluorescence spectroscopy
33. Q3 Principle and applications of luminometry
34. Q4 a. Select the correct alternative: (Any six) 6 marks
35. Q1 material that fluoresces when struck by a charged particle or high energy photon is known as
36. Q2 by negatron emission: Neutron --> + Negatron In process, changes occur in the atomic nucleus, and particles and/or electromagnetic radiation are emitted 4, In chromatography, separation. relies on the attraction between
37. Q5 can be used as a matrix in ion exchange chromatography
38. Q6 Relative molecular mass determination can be done by chromatography
39. Q7 exploits the unique property of extremely specific biological interactions to achieve separation and purification
40. Q8 In Affinity chromatography, select a ligand
    • b. poor stability d. do not displays group selectivity
41. Q9 In affinity chromatography, purified compound is recovered from the ligand by
    • a. by dilution c. by reloading sample
    • b. by water wash d. by either specific or non-specific elution
42. Q4 b. Describe the following questions: (Any Two) 14 marks
43. Q1 Principle, working, and applications of size exclusion chromatography
44. Q2 Steps in HPLC method development and add a note on any three parameters for
45. Q3 GM counters and their applications in the detection of radioactivity
46. Q5 Short notes on the following: (Any four) 20 marks
    • a. Virus purification and assays
    • b. Mode of action of macrolides
    • d. Principle of Raman spectroscopy
    • f. Autoradiography

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