BSc Biotechnology Sem V 2016 17 2016-17 INDUSTRIAL BIOTECH Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 a. Attempt the following (Any three) dependent cell lines? used for long term culture of attached one basic construction Principle for gas entrainer nozzle in a deep ‘State an advantage of using an airlift fermenter?
   • v) State an application of tower fermenter
   • vi) What is a packed bed fermenter used for? Answer the following ( Any two)
   • i) State the significance and methods of pH control in a fermenter
   • ii) Elaborate on the working and application of an airlift fermenter with an
   • iii) What is the significance of foam in a fermenter? How is it controlled?
   • iv) Diagrammatically explain the working of a bubble column fermenter
2. Q2 a- Doas directed ( Any three)
   • i) State any one criterion essential to be satisfied by a culture to be inoculated
   • ii) State one application of middle skimmings
   • iii) Define primary screening
   • iv) What is inoculum development?
   • v) What does the scale define? What is pitching? 2 b. Answer the following (Any two) 12
   • i) Discuss the use of secondary screening in search for novel metabolites
   • ii) Elaborate on the various aspects to be considered in the context of scale
   • iii) Explain the development of inocula for mycelia processes
   • iv) Write a note on primary screening 3 a. Name them (any three)
   • i) Starter culture used in beer manufacture
   • ii) Inoculum in Streptomycin production
   • iii) Fungi used in Citric acid production
   • v) Precursor used in Penicillin production
   • vi) Inert support used in an acetator 3 b. Discuss (any two) The production of red wine
   • ii) The fermentation of beta lactum antibiotic 4 a. Give examples of (any three)
   • i) Salts used in recovery of proteins by precipitation
   • v) Detergents used in cell
   • vi) Inorganic adsorbants used in 4 b. Elaborate on (any two) Any two types of continuous filters 12
   • ii) Cell aggregation and
   • iii) Cell disruption by liquid and solid shear
   • iv) Membrane Write short notes on (any three)
   • ii) Pressure measurement in a fermenter
   • iii) Inlet and exit gas analysis

Read from the scan above, so a character or two may differ. The scan is the original.