BSc Biotechnology Sem I 2019 20 Nov 2019-20 BIOTECH BIOTECHNOLOGY I Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 For Q and Q 4 attempt A and B OR C and D Do as directed (Any fifteen) 15 hi What are the bonds required to build a dsDNA molecule?
4. Q2 How glycosidic bond forms between ribose sugar and pyrimidine base
5. Q3 What is the basic requirement of an oligonucleotide to act as a primer?
6. Q4 Role of magnesium ions in replication What is holoenzyme
7. Q6 centrifugation was used in Meselson and Stahl’s experiment What is replication?
8. Q8 What are mutagens.
9. Q9 Role of photolyase in photoreactivation,
10. Q10 Name the enzymes involved in methyl-directed Mismatch repair
11. Q11 Photoreactivation occurs when an enzyme called 1s activated by a photon of light and splits the dimers apart
12. Q12 State true or false. AT > TA is transversion mutation
13. Q13 State true or false. Depurination is the loss of a purine from the DNA the bond hydrolyses between the base and the deoxyribose sugar
14. Q14 In Avery’s transformation experiment, the mixture of DNA and RNA treated with DNase which caused degradation To prove that the phage genetic material was made up of DNA and not protein, Hershey and Chase:grew cells of E. coli in media containing either a radioactive isotope of
15. Q16 Enlist key characteristic of a material responsible for hereditary
16. Q17 State feature of R type strain used in Griffith’s transformation experiment
17. Q18 Give an application of Reverse Transcriptase enzyme In genetic 19, Give one example of selectable marker present on plasmid vector 20, Define the term “Star activity” of restriction enzyme
18. Q2 A_ Elaborate the of Meselson and experiment to prove 8 marks
19. Q2 B What is DNA types and its significance in replication. 7 marks
20. Q2 C notes on semi-discontinuous DNA replication with diagram. Explain the mechanism of rolling circle replication, 07 8 marks
21. Q3 A Define mutation. Give classification of mutations. 8 marks
22. Q3 B Describe the action of 5-bromouracil as mutagenic agent 7 marks
23. Q3 C Explain Nucleotide Excision repair with suitable diagram on the action of intercalating agents the details that showed DNA to be the geneuc material of T2 bacteriophage 8 marks
24. Q4 B Diagrammatically elucidate the experiment that showed RNA 1s the genetic material in tobacco virus Explain the features of Phosphatase and Ligases enzyme which are used 08 in genetic engineering the features of restriction endonucleases 07 Write Short notes on any three of the following 1s 7 marks
    • a. Short note on bidirectional replication
    • b. Diagrammatically represent conservative, and dispersive type of replication
    • d. Shuttle Vectors

Read from the scan above, so a character or two may differ. The scan is the original.