BSc Biotechnology Sem I 2018 19 Dec 2018-19 BIOTECH BASIC LIFE SCIENCES II MICROBIAL TECHNIQUES Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 Do as directed. (Any 15)
2. Q1 What is decimal reduction time?
3. Q2 Give one example of elements used in culture media
4. Q3 Define- Acid fast bacteria
5. Q4 Explain the term ‘Phototrophs’
6. Q5 Define- Mordant
7. Q6 Define- Simple Staining
8. Q7 Explain the term “Pure culture’
9. Q8 Define- Compound Microscope
10. Q9 is a simple and rapid method for measuring growth on the basis of the turbidity of the What is chromogen?
11. Q11 Define- Numerical aperture
12. Q12 Give one example of Organisms that can reduce inorganic compounds as electron donors Tyndallization is also known as Define- Natural Dyes
13. Q15 Name the process of destructing microorganisms by burning into the flame of the Bunsen burner
14. Q16 Give one example of substance used to preserve cultures for cryopreservation
15. Q17 is traditionally used as germicidal agent in a form as
16. Q18 State whether true of false
17. Q19 one example of gaseous agent that has germicidal activity
18. Q20 The time taken for a population to double in the regular interval of time is called
19. Q2 A) What is the resolving power of a microscope? How can resolving power be increased? 8 marks
20. Q2 B) Name several differential staining techniques and describe the type of information each provides
21. Q2 C) Elaborate on different theories of staining. 8 marks
22. Q2 D) What are the underlying principle, advantage and disadvantage of Dark field microscope? 7 marks
23. Q3 A) List several different kinds of radiations used for destruction of microorganisms. Comment on mode of action of any two of its examples. (08)
24. Q3 B) Describe filtration as physical agent for control of micro organisms. 7 marks
25. Q3 C) List several halogens & compounds of halogens to control microbial population. Add a note on practical applications of any two such compounds. (08)
26. Q3 D) Explain in brief the usage and limitations of any four chemical agents for control of micro
27. Q4 A) Elaborate on significance of nutritional requirements of micro organisms with respect to Nitrogen, Carbon, Phosphorus, Sulphur, growth factors and trace elements. (08)
28. Q4 B) Explain the term pure culture. How would you get the pure culture from the mixed population by isolation technique? (07)
29. Q4 C) How would you enumerate micro organisms indirectly by determining the Nitrogen content and dry weight of the cells? (08)
30. Q4 D) What is cryopreservation? Explain in detail the method and advantages of cryopreservation
31. Q5 Write short notes on the following. (Any three) 15 marks
32. Q1 Acid and basic dyes
33. Q2 Functions of Fixative
34. Q3 Incineration
35. Q4 Alcohol as antimicrobial agent
36. Q5 Synchronous growth

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