BSc Biotechnology Sem I 2018 19 Dec 2018-19 BIOTECH BASIC BIOTECHNOLOGY II MOLECULAR BIOLOGY Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 Do as directed: (Any Fifteen) 15 marks
   • 1. What is the function of helicase?
   • 2. What are mutagens?
   • 3. Name the scientists who designed pBR 322
   • 4. Name the enzymes that removes supercoiling in replicating DNA ahead of the replication
   • 5. Name the microorganism in which SOS response is best studied
   • 6. DNA replication is
   • 7. The source of DNA ligase is
   • 8. mutagens act by inserting themselves between adjacent bases in one or both strands of the DNA double helix
   • 9. State whether following statement is True/ False: During DNA replication, nucleotides are added on to the 5' end of the growing DNA strand
   • 10. State true or False. AT > TA is a transversion mutation
   • 11. State true or False: Transduction is transfer of genetic material between bacterial cells via
   • 12. Give one example of organisms commonly used for cloning
   • 13.Give one example of chemical mutagen
   • 14. Replication occurs once every cell generation during phase of cell cycle
   • 15. Give the name of one marker gene
   • 16. Role of photolyase enzyme in photoreactivation
   • 17. Name the two radioactive isotopes used by Hershey and Chase in their experiment
   • 18. What is nonsense mutation?
   • 19. The enzyme that stitches Okazaki fragments together (along the lagging strand) is called
   • 20. Name one gene transfer technique for Prokaryotes
2. Q2 A] Explain initiation of replication in Escherichia Coli. 8 marks
3. Q2 B] Describe the Meselson Stahl experiment, and explain how it showed that DNA replication is semiconservative. 07
4. Q2 C] Explain with a suitable diagram model of reciprocal recombination. 8 marks
5. Q2 D] In detail explain rolling circle replication. 7 marks
6. Q3 A] Explain the term mutation and elaborate on overall classification of mutation
7. Q3 B] Describe the action of 5-bromouracil as mutagenic agent. 7 marks
8. Q3 C] Explain base pair substitution. Discuss types of base pair substitution mutation. 8 marks
9. Q3 D] In detail explain photo reversal DNA repair. 7 marks
   • A] Describe an experiment to prove that DNA is the genetic material. 08
   • B] Elaborate on the features of Type II Restriction Endonucleases. 07
10. Q4 C] Describe Griffith’s experiment to prove that DNA is the genetic material. 8 marks
    • D] Describe the features of the plasmid vector pBR 322. 07
11. Q5 Short notes (Any 3) 15 marks
    • a. Expression vectors
    • c. Role of Telomerase
    • d. Intercalating agents
    • e. Biological mutagens

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