BSc Biotechnology Sem I 2019 20 Nov 2019-20 BIOTECH BASIC LIFE SCIENCE II Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q5 Q 4 attempt A and B OR C and D directed (Any fifteen) 15
4. Q1 Objectives are attached to part of the compound microscope
5. Q2 Calculate the working distance of covered object in sharp focus when 3.5 turns are taken by fine adjustment knob Define the term: Resolving power of a microscope
6. Q4 The etiological agent of Tuberculosis in human s is
7. Q5 Define the term: Stain
8. Q6 Give one example of differential staining method Define the term: Mordent
9. Q8 Give an example of dry heat as means of controlling microorganisms
10. Q9 What method would be employed to sterilize oil?
11. Q10 State whether true or false: Absolute ethyl alcohol is more effective than Define the term: Disinfection What is another name for fractional sterilization?
12. Q13 Name the derivative of phenol which is marketed in a solution of glycerin
13. Q14 | State whether true or false: dessicated cultures of microorganisms remain viable for many years 1S. Define the term: Heterotroph
14. Q16 What is meant by the term: Enriched media?
15. Q17 What is a ‘pure culture’ in microbiology? The term used to describe the growth phase in which there is maximum secondary metabolite formation is
16. Q19 What is the use of Petroff-Houser chamber in microbiology?
17. Q20 any one cryoprotective agent used in the preservation of bacteria
18. Q2 A_ Describe construction and working of dark field illumination technique, Explain types of objectives. Add a note on its chief functions. 07 8 marks
19. Q2 C Define dye. Explain acidic and basic dyes with suitable examples. Describe the method of Ziehl-Neelsen staining. 07 Give a brief account on moist heat as a physical agent for controlling 08 8 marks
20. Q3 B are effective in controlling microorganisms. Justify. Give a brief account on halogens as a chemical agent of controlling 08 7 marks
21. Q3 D radiations effective in controlling microorganisms. 7 marks
22. Q4 A_ Explain the phenomenon of diauxic growth of bacteria. How would you ~ Isolate bacteria by the spread plate method? 07 8 marks
23. Q4 C_ Explain the concept of growth yield of bacterial cultures. 8 marks
24. Q4 D How would you — Enumerate bacteria by the colony count method? 7 marks
25. Q5 Write Short notes on any three of the following 15 marks
    • a. Theories of staining
    • b. Natural stains with examples
    • e. Lyophilization

Read from the scan above, so a character or two may differ. The scan is the original.