BSc Biotechnology Sem I 2022 2023 Dec 2023 BIOTECH. MOLECULAR BIOLOGY I Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 For Q2, Q3 and Q 4 attempt A and B OR C and D directed (Any fifteen) 15
3. Q1 , a specific set of sequences at the end of a linear chromosome, stabilizes the chromosome and is required for replication. 4
   • a. Centromere b. d. Ribosome In DNA, 5’ to 3’ phosphate linkages are called chromosome has the centromere at about the center, so the chromosome appears to have two approximately equal arms
4. Q4 Define the term ‘Genome’?
5. Q5 are regions internal to the simple telomeric sequences
6. Q6 What are two classes of nitrogenous bases present in DNA? is a pre-existing polynucleotide chain in DNA replication to which new nucleotides can be added
7. Q8 In DNA replication, exonuclease activity is a proofreading
8. Q9 The segments of untwisted single strands upon which the new strands of DNA are made are called the strands DNA replication, the proteins bind to the parental molecule at the origin of replication and wrap the DNA around them Primase activated by DNA helicases synthesizes a short required for initiation of DNA synthesis
   • d. exonuclease activity pa The RNA primers themselves do not remain as a part of the new DNA chain, they are removed and replaced with DNA by the action of
9. Q13 In eukaryotes, the enzyme serves a DNA repair function
10. Q14 plays an important role in the Mismatch repair mechanism
11. Q15 | AP endonuclease plays an important role in mechanism VCD FYBT SEMI MOLECULAR BIOLOGY-I 2%HRS 75MARKS Photoreactivation occurs when an enzyme called is activated by a photon of light and splits the dimers apart
12. Q17 is the point mutation occurs by substitution of purine by pyrimidine and vice versa
13. Q19 Give any TWO examples of viruses with DNA as a genetic material
14. Q20 Give any TWO examples of biological mutagens
15. Q2 A Elaborate the DNA double helix- Watson and Crick’s Model. Give details of chromosome banding techniques. (Any TWO) 07 8 marks
16. Q2 C Elaborate the packaging of DNA into chromosomes. 8 marks
17. Q2 D What is ‘nucleoside’? Draw structure of Cytosine (C) and Thymine (T). Elaborate on the experiment that Meselson and Stahl used to prove the 08 semiconservative mode of replication in prokaryotes 7 marks
18. Q3 B Explain why DNA replication as a whole is considered to occur in a semi- 7 marks
19. Q3 C Witha suitable model explain reciprocal recombination in detail. In detail explain the initiation of DNA replication in eukaryotes. 07 the mechanism of Base Excision Repair. 08 8 marks
20. Q4 B Define the term Explain following types mutations: 7 marks
21. Q4 C Describe the mechanism of Mismatch repair. 8 marks
22. Q4 D What are “Mutagens”? Describe action of base analogue with any ONE 7 marks
23. Q5 Short notes on any three of the following 15 marks
    • a. Histone and Non-histone proteins
    • b. Human Karyotype
    • d. Enzymes involved in prokaryotic DNA replication
    • e. Photoreversal Repair

Read from the scan above, so a character or two may differ. The scan is the original.