BSc Biotechnology Sem VI ATKT 2018-19 ATKT Biotech Molecular Biology & Bioinformatics Question Paper - Mumbai University | munotes

Questions asked in this paper

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1. Q1 a. Give the importance of ( any three) 3 marks
2. Q1 vaccines
   • ii) Primers
   • iii) VNTRs
   • b. Attempt the following (any two) 12
   • i) Comment on the advantages of genetic. immunization
   • ii) Justify: vaccinia virus is an ideal candidate as vector vaccines
   • iii) Give an account of Sanger’s method for DNA sequencing
   • iv) Diagrammatically explain Southern blotting
3. Q2 a. Do as directed (any three): 3 marks
4. Q1 Define: electroporation State the importance of cry protein Which is the gene coding for phytoene synthase in production of golden
   • iv) What is triparental mating?
   • v) is mini-binary vector?
   • vi) Define: microprojectile
   • b. Attempt the following (any two): 12
5. Q1 Elaborate on the application of recombination DNA technology in improving the quality of food
   • ii) Elaborate on protoplast fusion
   • iii) Discuss: Ti plasmid mediated vector system
   • iv) Write a note on the ballistic method of DNA delivery to create transgenic
   • Q.P. Code :32309
6. Q3 a. What do you understand by the following terms? (any three): 3 marks
   • ii) Pronucleus
   • v) recombination vi)
   • b. Elaborate the following (any two): 12
7. Q1 Establishment of transgenic mouse with engineered embryonic stem cells
   • ii) Construction of a lentivirus derived vector
   • iii) recombination system for inactivation a gene
   • iv) Transgenic fish as biosensors for environmental pollution
8. Q4 a. Explain the following (any three): 3 marks
   • iii) NCBI
   • iv) Browser
   • vi) FASTA
   • b. Answer the following (any two): 12
9. Q1 Give a detailed account of scope and applications of Bioinformatics is internet? Explain the role of played by internet in Tools used for sequence alignment Q:5 Write short notes on (any three): 15
10. Q1 Applications of transgenic animals
    • ii) BLAST
    • ii) Application of PCR
    • iv) . vaccine

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