BSc Biotechnology Sem VI 2016 17 2016-17 Biotech SemVI Moleculaer Biology Question Paper - Mumbai University | munotes

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1. Q2 All questions carry equal marks
2. Q1 a. directed ( any three ) 3 marks
   • i. enzyme is used for digestion of 8 —globin gene in cell trait
   • ii. Taq polymerase extracted from organism is used in PCR
   • iii. What is the difference between dNTP and ddNTP?
   • iv. State any two strategies used for insertion of DNA into the host cell for genetic immunization
   • v. Explain the term — Peptide vaccine
   • vi. State any two advantages of Vaccinia virus as a vector for vaccine preparation
   • b. Attempt the following (any two) 12
   • i. Comment: DNA fingerprinting has many applications
   • ii. Discuss steps involved in PCR cycle
   • iii. Explain the preparation of
   • iv. Schematically explain the preparation of subunit vaccine against HSV
3. Q2 a. Give an example of the following ( any three ) 3 marks
   • i. Agrobacterium species
   • iii. Types of pTi
   • v. Plants used for preparation of edible vaccines
   • vi. Transgenes incorporated in normal rice to produce Golden rice
   • b. Answer the following (any two ) 12
   • i. Discuss edible vaccines with.a suitable example
   • ii. Describe the transfer of T-DNA in plants
   • iii. of ‘Electroporation as a method of producing transgenic plants’
   • iv. What is the mode of action of Cry protein?
4. Q3 a. Define the following (any three ) 3 marks
   • i. Stem cell
   • v. Antifreeze protein
   • vi. Cloning
   • b. Give an account of the following (any two ) 12
   • i. Transgenic a means to improve fish aquaculture Production of transgenic mice by DNA microinjection
   • iii. Cre —loxP system for regulating transgene expression
   • iv. Applications of transgenic animals
   • Q.P. Code :01207
5. Q4 a. Explain the following terms (any three) 3 marks
   • ii. URL
   • iv. PIR
   • vi. HTML
   • b. Answer the following (any two) 12
   • i. Discuss the scope and application of Bioinformatics Define sequence similarity searching. Describe the tools used in similarity searching
   • iii. Give an account of role of computers in Bioinformatics
   • iv. What is WWW? Explain its importance in Bioinformatics
6. Q5 Write short notes on ( any three ) 15 marks
   • a) Diagnosis of sickle cell anemia using RFLP
   • d) vector system
   • e) Any one test to ensure the site specific integration of a target gene

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