BSc Biotechnology Sem V 2019 2020 Oct 2020 BIOTECH GENOMES & MOLECULAR BIOLOGY 14.10.19 Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 Use of log tables and non-programmable calculator is allowed
3. Q1 a. Doas directed: (Any Six) 6 marks
4. Q1 is disarmed Ti plasmid?
5. Q2 significance of protoplast
6. Q3 What is ‘Marker gene’?
7. Q4 the voltage- time conditions used for electroporation
8. Q5 What is ‘Agroinfection’?
9. Q6 State an advantage of Ti plasmid based vectors for plant transgenesis
10. Q7 State an advantage of using viral vector for plant transgenesis
11. Q8 Define Opines
12. Q9 State the role of Gemini virus
13. Q1 b. Answer the following questions: (Any Two) 14 marks
14. Q1 Explain the use of ‘cointegrate vector system’ using a neat labeled
15. Q2 Describe the mechanism of transfer of T-DNA in plants
16. Q3 What is the need for seed quality protein improvement? How can it be achieved using transgenic technology? Explain giving example instructed: (Any Six) 06
17. Q1 Define Karyogamy State the use of Xenomouse
18. Q3 Name any one vector used for animal cell State the role of cre gene What do you mean by Pharming? Give the significance of Biosensor: gfp gene codes for Name the compound used for positive and negative selection of Explain the term Quiescent state
19. Q2 b. Give an account of: (Any Two) 14 marks
20. Q1 Embryonic stem cell method of producing transgenic mice
21. Q2 Microinjection method for producing transgenic mice
22. Q3 Positive and negative selection method for transgenic mice
23. Q3 a. Answer the following objective questions as directed: (Any Six) 6 marks
24. Q1 One application of S1 nuclease
25. Q2 Cloning capacity of plasmid vector
26. Q3 The acronym HART stands for
27. Q4 library which provides information on the types of mRNA in a particular cell at a particular time (select the correct answer):
28. Q5 Chromosome Walking
29. Q7 the significance of MCS
30. Q8 True or False: Genomic library helps to study the regulatory genes
31. Q9 Define alpha complementation
32. Q3 b. Attempt the following questions: (Any Two) 14 marks
33. Q1 Explain Northern Blotting
34. Q2 Describe a method to screen genomic library
35. Q3 How would you use a cosmid as a cloning vector?
36. Q4 a. the following objective questions as instructed: (Any Six) State true or false: ZFNs are artificial restriction enzymes generated by fusing DNA-binding and DNA cleaving domains 6 marks
37. Q2 What is the use of piperidine? Give full form of CRISPR Luciferase is used in method to identify the order of
38. Q6 State true or false: CRISPR/Cas 9 system induces single stranded break Which method of DNA sequencing uses florescence labeling?
39. Q8 Name the protein domain that recognizes a single bp in DNA sequence
40. Q9 true or false: Maxam Gilberts's method uses dideoxynucleotides
41. Q4 b. Discuss the following: (Any Two) 14 marks
42. Q1 Components and applications of RNAi technology
43. Q2 Sanger's method of DNA sequencing
44. Q3 Use of ZFNs and in genome editing
45. Q5 Write Short notes on the following: (Any Four) 20 marks
    • a. Biolistic method
    • b. Reporter genes
    • c. Cloning sheep by nuclear transfer method
    • d. Cre-loxP recombination system for activating a gene
    • e. Chromosome jumping
    • f. Maxam Gilbert's method of sequencing

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