BSc Biotechnology Sem I 2017 18 2017-18 Biotech Microbial Technology Question Paper - Mumbai University | munotes

Questions asked in this paper

• 1. Attempt all questions

1. Q2 All questions carry equal marks
2. Q4 For Q 2,Q 3 and Q 4 attempt A and B OR C and D Doas directed: (Any fifteen) 15
3. Q1 Distortion
4. Q2 Achromatic condenser Give one example of a Mordant
5. Q4 one example of an Azo dye
6. Q5 the significance of an Ocular
7. Q6 in the blank: Number of complete turns of a micrometer screw indicates measure of of an objective. (working distance/ resolving power)
8. Q7 Name an axillary group in a dye which imparts salt forming properties to the Give one example
9. Q8 Equipment working on steam sterilization technique
10. Q10 Disinfectant used in Biotechnology laboratory
11. Q11 Chemical sterilant
12. Q13 Indicator organism for checking efficiency of autoclave How would you sterilize heat labile liquids? Define : Complex medium the blank: Preservation of micro organisms in liquid nitrogen at is called Fill in the blank: During the phase, the microorganisms divide and double in number at regular intervals
13. Q19 one example of a Bacterium that can assimilate atmospheric Nitrogen
14. Q20 Give one example of a Differential media
    • Q. P. Code: 20665 Describe the functions and different types of objectives used in a compound 08 Explain the difference between ‘acidic’ and ‘basic’ stains with examples and give 07 their applications. Describe what is meant by “differential staining'?
15. Q2 C the differences between “Bright field’ and. ‘Dark field' microscopy? State the applications of dark field microscopy 8 marks
16. Q2 D_ Explain the terms “Gram and bacteria giving an example. Explain the method for their differential staining Explain the role of Ionizing and non-ionizing radiation in sterilization using examples. 08 7 marks
17. Q3 B State the mode of action of detergents and emulsifiers on microbes. Describe the characteristics of an ideal disinfectant. Give three examples. 08 Discuss the mode of action of gases on microbes. 07 7 marks
18. Q4 What do you mean by pure culture? Enlist the different methods used to obtaina pure culture. Explain any one method to obtain pure culture What is continuous culture system? Describe the types of continuous culture system. 07 8 marks
19. Q4 C on the measurement of cell mass for enumerating the bacteria. Enlist and explain the methods involved to preserve and maintain pure cultures of 07 Write Short notes on: (Any three) 15 8 marks
    • a. Mechanism of acid-fast staining Halogens as skin disinfectant Types of culture media
    • e. Phases of growth curve

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