BSc Biochemistry Sem VI 2018 19 May 2018-19 BIO CHEM BIOCHEMISTRY PAPER I METABOLISM & ANALYTICAL TECHNIQUES II Question Paper - Mumbai University | munotes

Questions asked in this paper

• (2) Figures to the right indicate Full Marks

1. Q1 (A) Choose the most appropriate answer (any three) : 3 marks
   • (i) A fatty acid with 16 carbon atoms will undergo cycles of beta oxidation and results in acetyl CoA molecules
   • (u) The energy requiring step of fatty acid oxidation is catalysed by
   • (iii) All are conditions of ketosis except
   • (a) starvation (tv) The conversion of acetyl CoA to malonyl CoA requires which of the
   • (v) Which of the following statements best describes the fatty acid synthase
   • (a) of similar units
   • (b) It is composed of 7 different proteins
   • (c) It dissociates into eight different proteins
   • (vi) NADPH required for fatty acid synthesis can be obtained from
   • Q.P. Code : 04583
   • (B) Attempt any one of the following : 2
   • (i) Describe Knoop's contribution to fatty acid metabolism State the difference between beta oxidation of odd numbered and even
   • (C) Attempt any one of the following : 4
   • (ii) Write a note on carnitine shuttle
   • (D) Answer any one of the following : 6
   • (i) Schematically represent mitochondrial reactions of beta-oxidation of a saturated fatty acid. How many molecules of ATP would be generated C20 saturated fatty acid was metabolised?
   • (ii) Elaborate on ketone bodies
2. Q2 (A) Choose the most appropriate answer (any three) : 3 marks
   • (1) T3 and T4 combine with and are transported in the blood
   • (c) Thyroglobulin
   • (ii) A child suffers from an amino acid deficiency because of which he has low levels of melatonin. The deficient amino acid is is the compound shared by TCA and Urea Cycle?
   • (iv) A15 year old boy with Type | diabetes faints on administration of insulin He is administered glucagon and he recovers. Which enzyme did glucagon
   • (a) Glycogen synthase
   • (b) Glycogen phosphorylase
   • Q.P. Code : 04583
   • (v) Which of the following is NOT a water soluble hormone?
   • (vi) A55 year old man has been diagnosed with liver cirrhosis, ammonia is not getting detoxified and can damage the brain. Which one of the following amino acids can be used to store ammonia in a non toxic form?
   • (B) Define Explain the term any one: 2
   • (C) Write a note on any one of the following : 4
   • (i) Synthesis of thyroid hormones
   • (D) Write detailed answers to any one of the following : 6
   • (i) Describe the physiological role of any two hormones involved in carbohydrate
   • (ii) Depict Krebs-Henseleit cycle and give its significance in humans
3. Q3 (A) Choose the most appropriate answer (any three) : 3 marks
   • (i) The light source is usually a lamp for visible region
   • (ii) In Beer's law, the intensity of transmitted light decreases exponentially as
   • (a) length of absorbing medium increases
   • (b) length of absorbing medium decreases
   • (c) concentration of absorbing medium decreases
   • (iii) The visible range of electromagnetic spectrum ranges between to
   • Q.P. Code : 04583
   • (iv) What should be the molar extinction of the solute which shows absorbance of 0.3 at 6.0 mM concentration and path length of 0.01 m?
   • (v) Estimation of protein with alkaline copper sulpahate is dependent on complex formation with groups
   • (vi) A blank contains all except
   • (B) Define Explain the term any one: 2
   • (C) Write a note on any one of the following : 4
   • (1) Colorimeter Types of centrifuges
   • (D) Write detailed answers to any one of the following : 6
   • (i) Professor Geeta seeks help to find the concentration of proteins and sugars in her plant extract. Write an informative note to solve her problem
   • (ii) Elaborate on centrifugation techniques based on density gradient
4. Q4 (A) Choose the most appropriate answer (any three) : 3 marks
   • (i) Full form of TEMED is Agar consists of and
   • (a) galactose, glucose
   • (b) galactose, pectin
   • Q.P. Code : 04583
   • (iv) Gases produced on cathode and anode during electrophoresis are
   • (v) buffer can be used for separation of nucleic acid in electrophoresis
   • (vi) The function of beta mercaptoethanol is
   • (a) to give negative charges to amino acids in the proteins
   • (b) for the oxidation of disulfide bonds in the proteins
   • (c) for the reduction of disulfide bonds in the proteins
   • (B) Attempt any one of the following : 2
   • (i) State the utility of APS in electrophoresis
   • (ii) Describe the underlying principle of electrophoresis
   • (C) Write a note on any one of the following : 4
   • (i) Electrophoretic mobility of anion
   • (D) Answer any one of the following : 6
   • (i) Write a note on applications of electrophoresis
   • (ii) Justify : "Electrophoresis is a technique that uses diverse materials for running
5. Q5 (A) Write short note on any one of the following : 3 marks
   • (ii) Rate limiting step of fatty acid catabolism
   • (B) Write short note on any one of the following : 3
   • (i) Differentiate between type-I and type-II diabetes mellitus
   • (ii) Write briefly on transaminases
   • Q.P. Code : 04583
   • (C) Answer in brief any one of the following : 3
   • (ii) Schematically represent the separation of cell organelles using centrifugation
   • (D) Answer in brief any one of the following : 3
   • (i) Differentiate betwen stacking and resolving gel
   • (ii) Write a note on dyes used for visualization of biomolecules seperated by
   • (E) State True or False (any three) : 3
   • (ii) Oxytocin is a pitutary hormone
   • (iii) Catabolism of fatty acids leads to formation of malonyl CoA
   • (iv) Centripetal force acts away from the axis of rotation
   • (v) Molar extinaction co-efficient has units mM/ min
   • (vi) SDS is used as a cross-linking agent in electrophoresis

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