BSc Biochemistry Sem VI 2018 19 May 2018-19 BIO CHEM BIOCHEMISTRY I METABOLISM AND ANALYTICAL TECHNIQUES II Question Paper - Mumbai University | munotes

Questions asked in this paper

1. Q1 All the questions are compulsory. Choice is internal
2. Q2 Figures to the right indicate full marks
3. Q3 All questions carry equal marks
4. Q5 Use of simple calculator is allowed
5. Q1 A) Fillin the blanks (any three): 3 marks
   • ii) Enzymes for the fatty acid oxidation are present in the lii) is the end product of beta oxidation of even carbon saturated fatty
   • v) Synthesis of fatty acid is termed as
6. Q1 B) Answer in brief any one of the following: 3 marks
   • i) Discuss the contribution of Knoop to the field of lipid metabolism
   • ii) Briefly explain the fate of glycerol obtained on breakdown of triglycerides Attempt any one of the following: 6
   • i) With the help of a schematic representation, explain how the carnitine shuttle plays an important role in determining the rate of beta oxidation. Emphasize on its significance in lipolysis
   • ii) Explain in detail the structure and function of the multi-enzyme complex involved in synthesis of fatty acids
7. Q1 D) Answer any one of the following: 8 marks
   • i) With the help of structures and enzymes involved, explain the oxidation of Eicosanoic acid (C-20). Also, elaborate on the bioenergetics of the same
   • ii) ‘Diabetic individual usually excrete ketone bodies in their urine.’ Elaborate in detail on the formation and utilization of ketone bodies by a diabetic individual Fillin the blanks (any three): 3
8. Q1 Aspartate Transaminase is the other name for li) is regenerated at the end of urea cycle lil) functions as the secondary messenger
   • iv) Congenital hypothyroidism is also known as
   • v) hormone causes suppression of immune system Answer in brief any one of the following: 3
9. Q1 Justify: Amino acid degradation is different from other catabolic processes
   • ii) Explain the sequential process of insulin biosynthesis
10. Q2 C) Differentiate between any one of the following : 6 marks
    • ii) Oxidative and non-oxidative deamination
11. Q2 D) Write detailed answers to any one of the following: 8 marks
    • i) Discuss in detail thyroid hormones under the following headings: (a) chemistry
    • ii) With the help of structures, explain the final pathway of protein degradation. Also, mention the cellular location and trace the origin of the two nitrogen atoms
12. Q3 A) the blanks (any three): 3 marks
13. Q1 wavelength range is associated with visible colorimeter
    • ii) Desk top centrifuge has a capacity of ml
    • iii) The first fraction obtained at 600 rpm in differential centrifugation of liver
    • iv) Unit of sedimentation coefficient is
    • v) Rate of sedimentation is proportional to viscosity of a medium
14. Q3 B) Attempt in brief any one: 3 marks
    • i) Explain the basic principle of sedimentation
    • ii) A series of 3 colored glass plates of equal thickness are placed in.a light beam Each sheet absorbs. one quarter of the light incident upon it. What is the intensity of the light transmitted by the third glass plate?
15. Q3 C) Attempt any one of the following : 6 marks
    • i) Compare and contrast: Rate zonal and isopycnic centrifugation. Name any two examples of compounds used as density gradient materials
    • ii) State Beer-Lambert’s law and add a note in detail about deviation and limitation of the same
16. Q3 D). Write detailed answers of any one of the following: 8 marks
    • i) Write a detailed note on analytical ultracentrifugation
    • ii). Explain construction of spectrophotometer with respect to following
    • a) Source of light b) wavelength selector Fillin the blanks (any three): 3
17. Q1 dye is used to stain nucleic acids
    • ii) High molecular weight particles travel in a supporting medium with low porosity than low molecular weight components Agarose gel has molecular sieving effect
    • iv) a/an detergent
    • v) Carbohydrates can be separated by electrophoresis using buffer
18. Q4 B) Answer in brief any one of the following: 3 marks
    • i) Give a brief account on Native PAGE
    • ii) An inert supporting medium is chosen for electrophoresis. How does it influence the movement of sample?
19. Q4 C) Attempt any one of the following: 6 marks
    • i) Explain the effect of 1) composition 2) ionic strength and 3) pH of the buffer on the rate of migration of sample in electrophoresis
    • ii) Elaborate on the characteristics and applications of 1) paper and 2) cellulose acetate as supporting media
20. Q4 D) Answer any one of the following: 8 marks
    • i) State True or False, giving reason: Pore size can be controlled in polyacrylamide gels. With the help of reaction explain the formation of the gel including the role of ammonium persulphate and TEMED
    • ii) Justify: Electrophoresis has wide array of applications
21. Q5 A) Answer any four of the following: 16 marks
    • i) Discuss the formation of malonyl CoA. Elaborate on its significance to lipid
    • i) Describe the pathophysiological conditions leading to ketone bodies and explain their effect on human physiology
    • ii) Explain the physiological functions of glucocorticoids
    • ii) In detail, elaborate on the reaction mechanism of transamination reactions
    • iii) Write on general properties of gradient material
    • iii) How would you estimate the unknown concentration of protein using colorimetric
    • iv) Briefly explain the working of type of electrophoresis that uses two different gels
    • iv) Describe the principle of electrophoresis. Give any one example each of a buffer and a dye used for separation of protein State true or false (any four): 4
    • i) max is independent of concentration for a particular biomolecule
    • ii) Svedberg (S) is the unit in centrifugation ili) The coenzyme required for oxidative deamination is pyridoxal phosphate
    • iv) acid synthase complex has a structural and functional division
    • v) Intact protein molecules are separated using SDS-PAGE
    • vi) pH of the stacking gel is 2 units higher than that of separating gel

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